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Phase III Operations: The University is open for expanded research operations; only authorized personnel will be admitted on campus. More info here.

Sample Submission

For most sample submissions it is beneficial to discuss the experiment, goals and expectations and we therefor encourage users to arrange, ahead of time, an appointment.  For peptide synthesis we suggest to fill out our Custom Peptide and Peptide Library Submission Form.

For a user opting to ship/mail samples, please use our mailing address and share tracking number if available.

All samples are logged and labelled with a tracking number which typically begins with P (for peptides) or MS (for mass spec experiments) followed by a number where the two first digits indicate the Fiscal Year. Internal users can track the progress/status for submitted requests here. When contacting the PRC re. past analyses, please  refer to the P or MS number.

Orange Cap vials labelled with PRC tracking numbers.

All samples received at the PRC will be labelled with a unique number.

 

 

 

 

 

 

For external users we create an account and will need name, email and phone numbers of user, PI and administrator as well as your lab address. We will generate a cost estimate after discussing your project and the estimate should be used to generate a Purchase Order (PO). We can not accept checks or credit cards.

Currently (December 1, 2019), the median turn-around time for mass spec based analysis is 8 days with an average of 2 weeks but processing time is project dependent. Turn-around time for peptide synthesis requests is 4-5 weeks.

 

Order of analysis/injection

Nano-LC systems (very often used in proteomics) are difficult to fully clean/clear after each injection because of the low flow and 2-digit micrometer columns. Therefore, when sample amounts are high or if some peptides are particular hydrophobic, carry-over is a possibility. Carry-over refers to the detection of a
molecule from the previous injection. For nano-LC carry-over can be as high as 5% – but for higher flow LC (>100 uL/min) this is much less of a problem. To minimize potential carry-over for proteomics samples we ask that you consider the order of injection. For example:

  • Analyze control before treatment IF you expect specific proteins or PTMs to only be present after treatment.
  • When comparing isoforms separated by SDS-PAGE, analyze the lower bands before the higher bands.
  • When amounts are very different between samples, analyze in order of increasing amounts.

At times the order can be difficult to decide but the PRC will be able to help you assess your samples.