Instruments
BD LSR (BD LSRIIs and BD LSR-Fortessa)
Former (Retired) Instruments:
For cell sorting, the FCRC at RU is equipped with three BD FACSAria Cell Sorters from BD Biosciences and one MA900 cell sorter from Sony Biotechnology. All instruments provide high-speed, high purity multi-laser cell sorting into up to four tubes and are also capable of sterile sorts of single cells directly into culture plates. BD FACSAria cell sorters are operated by FCRC staff only. Sony MA900 can be operated by researchers with full time (24/7/365) access, after completion of the required training.
For analysis purposes, the FCRC at RU is equipped with six benchtop analyzers, which cover the wide range of researcher’s needs:
The spectral Analyzers Cytek Aurora have five lasers (355, 405, 488, 561 and 640 nm) and 64 fluorescence channels. It has a unique capability of measuring the entire emission spectra of the fluorescent dyes excited by multiple lasers installed on the instrument. The full spectrum capture enables the use of the novel unmixing algorithms for the further data analysis. Till now up to 40 different fluorescent labels could be resolved, even when multiple markers are co-expressed on the same cell.
We also have four benchtop alignment-free multipurpose advanced analyzers. Three of them from BD Biosciences: BD LSRII-1, BD LSRII-2 and BD LSR-Fortessa. The laser configurations on the LSR instruments allow for analysis of cell samples stained with 488 nm, 640 nm, 355 nm (UV), 405 nm, 445 nm and 561 nm excited dyes. A number of applications, including the multicolor analysis of cell phenotype, gene expression, cell cycle, and others may be performed. ThermoFisher Attune NxT is an advanced analyzer equipped with four lasers (405, 488, 561 and 637 nm) and 14 fluorescence channels. Using the unique acoustic focusing feature, the Autosampler enables Attune to run samples tubes or in plate formats, including 96-well, 384-well, and deep-wells with the high volume intake.
After orientation trainings (Virtual Classroom, “Beyond the Basics” Class, Pre-Hands-On Consultation and Instrument Hands-On Training), Cytek Aurora, BD LSRII-1, BD LSRII-2, BD LSR-Fortessa and ThermoFisher Attune NxT are available for use directly by the investigators.
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