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Instruments

 

 

For cell sorting, the FCRC at RU is equipped with three BD FACSAria Cell Sorters from BD Biosciences and one MA900 cell sorter from Sony (Coming Soon). All instruments provide high-speed, high purity multi-laser cell sorting into up to four tubes and are also capable of sterile sorts of single cells directly into culture plates. BD FACSAria cell sorters are operated by FCRC staff only. Sony MA900 can be operated by researchers with full time (24/7/365) access, after completion of the required training.

For analysis purposes, the FCRC at RU is equipped with seven benchtop analyzers, which cover the wide range of researcher’s needs:

Amnis ImageStream-X (IMAGE Analyzer) is equipped with 488 nm, 405 nm, 561 nm and 658 nm lasers and ten fluorescent detectors. This unique technology allows to put together data for cell morphology, fluorescence localization, and population statistics for a broad range of applications. ImageStream-X is operated by FCRC staff only.

Cytek Aurora (SPECTRAL Analyzer) has five lasers (355, 405, 488, 561 and 640 nm) and 64 fluorescence channels. It has a unique capability of measuring the entire emission spectra of the fluorescent dyes excited by multiple lasers installed on the instrument. The full spectrum capture enables the use of the novel unmixing algorithms for the further data analysis. Till now up to 40 different fluorescent labels could be resolved, even when multiple markers are co-expressed on the same cell.

We have five benchtop alignment-free multipurpose analyzers. Four of them from BD Biosciences: three Advanced Analyzers (BD LSRII-1, BD LSRII-2 and BD LSR-Fortessa) and one Basic Analyzer BD Accuri® C6. The laser configurations on the LSR instruments allow for analysis of cell samples stained with 488 nm, 640 nm, 355 nm (UV), 405 nm, 445 nm and 561 nm excited dyes. A number of applications, including the multicolor analysis of cell phenotype, gene expression, cell cycle, and others may be performed. The BD Accuri® C6 is equipped with a Blue (488nm) and a Red (640nm) lasers and four fluorescence detectors optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. It supports the high throughput and fully automated acquisition from standard 12 x 75-mm tubes, to 48- and 96-well plates and deep-well plates. ThermoFisher Attune NxT is an advanced analyzer equipped with two lasers (488 and 561 nm) and seven fluorescence channels. Using the unique acoustic focusing feature, the Autosampler enables Attune to run samples tubes or in plate formats, including 96-well, 384-well, and deep-wells with the high volume intake.

After orientation training (Virtual Classroom, “Beyond the Basics” Class and Instrument Hands-On Training), Cytek Aurora, BD LSRII-1, BD LSRII-2, BD LSR-Fortessa, ThermoFisher Attune NxT and BD Accuri C6 are available for use directly by the investigators.

Click to enlarge.

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