Skip to main content
!
Phase III+: The University is open for expanded research operations; only authorized personnel will be admitted on campus. More info here.

Virtual Classroom for Pre-Sort Consultation

 

BD Biosciences logo

 Overview5:01

  • Define the term flow cytometry.
  • Describe the types of samples suitable for a flow cytometer.
  • Explain the properties that a flow cytometer can measure.
  • Identify the three subsystems of a flow cytometer.

 Optical measurements – 9:48

  • Describe the factors that affect light scatter.
  • Explain how light scatter and fluorescence can be used to detect populations.
  • Describe the use of excitation and emission spectra.
  • Explain what compensation is and why it is necessary for multicolor analysis.

 Sorting – 4:25

  • Describe the purpose of sorting cells or particles.
  • Explain how particle size affects the nozzle size and sheath pressure selection.
  • Describe the steps in droplet sorting.

 

 

bio-rad_logo

Optimize your Flow Cytometry-Best Practices for Sample Preparation, Staining and Analysis

Bio-Rad_Optimize your Flow Cytometry_Crop