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Instruments

 

For cell sorting, the FCRC at RU is equipped with three BD FACSAria Cell Sorters from BD Biosciences. All instruments provide high-speed, high purity multi-laser cell sorting into up to four tubes and are also capable of sterile sorts of single cells directly into culture plates. Cell Sorters are operated by FCRC staff only.

For analysis purposes, the FCRC at RU is equipped with seven benchtop analyzers, which cover the wide range of researcher’s needs:

Amnis ImageStream-X (IMAGE Analyzer) is equipped with 488 nm, 405 nm, 561 nm and 658 nm lasers and ten fluorescent detectors. This unique technology allows to put together data for cell morphology, fluorescence localization, and population statistics for a broad range of applications. ImageStream-X is operated by FCRC staff only.

Cytek Aurora (SPECTRAL Analyzer) has four lasers (488, 561, 640 and 405 nm) and 48 fluorescence channels. It has a unique capability of measuring the entire emission spectra of the fluorescent dyes excited by multiple lasers installed on the instrument. The full spectrum capture enables the use of the novel un-mixing algorithms for the further data analysis. At least 23 different fluorescent labels could be resolved, even when multiple markers are co-expressed on the same cell.

We have five benchtop alignment-free multipurpose analyzers. Four of them from BD Biosciences: three Advanced Analyzers (BD LSRII-1, BD LSRII-2 and BD LSR-Fortessa) and one Basic Analyzer BD Accuri® C6. The laser configurations on these instruments allow for analysis of cell samples stained with 488 nm, 640 nm, 355 nm (UV), 405 nm, 445 nm and 561 nm excited dyes. A number of applications, including the multicolor analysis of cell phenotype, gene expression, cell cycle, and others may be performed. ThermoFisher Attune NxT is equipped with two lasers (488 and 561 nm) and seven fluorescence channels. Using the unique acoustic focusing feature, the Autosampler enables Attune to run samples tubes or in plate formats, including 96-well, 384-well, and deep-wells with the high volume intake.

After orientation training (Virtual Classroom“Beyond the Basics” Class and Instrument Hands-On Training), Cytek Aurora, BD LSRII-1, BD LSRII-2, BD LSR-Fortessa, BD Accuri C6 and ThermoFisher Attune NxT are available for use directly by the investigators.

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FCRC instruments specifications and comparison