Flow Cytometry Web-Based Training from BD Biosciences

1. Overview – 5:01

• Define the term flow cytometry.
• Describe the types of samples suitable for a flow cytometer.
• Explain the properties that a flow cytometer can measure.
• Identify the three subsystems of a flow cytometer.

2. Fluidics – 3:30

• Describe the functions of the fluidics system.
• Explain hydrodynamic focusing.
• Describe how sample pressure affects flow rate and data resolution.

3. Optics – 7:48

• Describe the functions of the optics system.
• Discuss the role of lasers in BD cytometers.
• Explain how filters route light to appropriate detectors.

4. Electronics – 6:59

• Describe how the electronics system converts light signals into numerical data.
• Explain how laser time delays ensure that signals from multiple lasers are attributed to the correct event.
• Explain how and why thresholds are used.

5. Optical measurements – 9:48

• Describe the factors that affect light scatter.
• Explain how light scatter and fluorescence can be used to detect populations.
• Describe the use of excitation and emission spectra.
• Explain what compensation is and why it is necessary for multicolor analysis.

6. Data Analysis – 8:54

• Describe how data is saved and displayed.
• Explain the purpose of gating.
• State examples of data analysis methods.

7. Sorting – 4:25

• Describe the purpose of sorting cells or particles.
• Explain how particle size affects the nozzle size and sheath pressure selection.
• Describe the steps in droplet sorting.