Skip to main content

Flow Cytometry Web-Based Training from BD Biosciences

BD Biosciences logo

1. Overview5:01

  • Define the term flow cytometry.
  • Describe the types of samples suitable for a flow cytometer.
  • Explain the properties that a flow cytometer can measure.
  • Identify the three subsystems of a flow cytometer.

2. Fluidics – 3:30

  • Describe the functions of the fluidics system.
  • Explain hydrodynamic focusing.
  • Describe how sample pressure affects flow rate and data resolution.

3. Optics – 7:48

  • Describe the functions of the optics system.
  • Discuss the role of lasers in BD cytometers.
  • Explain how filters route light to appropriate detectors.

4. Electronics – 6:59

  • Describe how the electronics system converts light signals into numerical data.
  • Explain how laser time delays ensure that signals from multiple lasers are attributed to the correct event.
  • Explain how and why thresholds are used.

5. Optical measurements – 9:48

  • Describe the factors that affect light scatter.
  • Explain how light scatter and fluorescence can be used to detect populations.
  • Describe the use of excitation and emission spectra.
  • Explain what compensation is and why it is necessary for multicolor analysis.

6. Data Analysis – 8:54

  • Describe how data is saved and displayed.
  • Explain the purpose of gating.
  • State examples of data analysis methods.

7. Sorting – 4:25

  • Describe the purpose of sorting cells or particles.
  • Explain how particle size affects the nozzle size and sheath pressure selection.
  • Describe the steps in droplet sorting.