1. Overview – 5:01
- Define the term flow cytometry.
- Describe the types of samples suitable for a flow cytometer.
- Explain the properties that a flow cytometer can measure.
- Identify the three subsystems of a flow cytometer.
2. Fluidics – 3:30
- Describe the functions of the fluidics system.
- Explain hydrodynamic focusing.
- Describe how sample pressure affects flow rate and data resolution.
3. Optics – 7:48
- Describe the functions of the optics system.
- Discuss the role of lasers in BD cytometers.
- Explain how filters route light to appropriate detectors.
4. Electronics – 6:59
- Describe how the electronics system converts light signals into numerical data.
- Explain how laser time delays ensure that signals from multiple lasers are attributed to the correct event.
- Explain how and why thresholds are used.
5. Optical measurements – 9:48
- Describe the factors that affect light scatter.
- Explain how light scatter and fluorescence can be used to detect populations.
- Describe the use of excitation and emission spectra.
- Explain what compensation is and why it is necessary for multicolor analysis.
6. Data Analysis – 8:54
- Describe how data is saved and displayed.
- Explain the purpose of gating.
- State examples of data analysis methods.
7. Sorting – 4:25
- Describe the purpose of sorting cells or particles.
- Explain how particle size affects the nozzle size and sheath pressure selection.
- Describe the steps in droplet sorting.