Considerations When Booking Sort Time

Considerations when Booking Sort Time for Bulk Sorting

• Setup time – First Experiment 30 min; Consequent Experiments 10 min
  • Check settings
  • Dead Cell Exclusion (DCE) Dye titration (First experiment only)
  • Compensation
  • Gating
• Sorting time calculated by cell numbers for certain nozzle size (take in account cell concentration)
• Consider number of samples to be run. Add 5 minutes for the sample switch

Considerations when Booking Sort Time for Plates Sorting

• Setup time – First Experiment 30 min; Consequent Experiments 10 min
  • Check settings
  • Dead Cell Exclusion (DCE) Dye titration (First experiment only)
  • Compensation
  • Gating
• Sorting time calculated by total (for all the samples) number of plates to be sorted (with 60 wells per plate) multiplied by 5-7 minutes per plate
• Consider number of samples to be run. Add 5 minutes for the sample switch