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Bead Compensation Controls

 

Reason to use BD FACSComp Bead as Single Stain Controls:

  • Cell numbers are limiting
  • Antigen is dimly expressed
  • Antibody has low affinity to receptor
  • Positive population is rare
  • Use tandem dyes

Note: Single color beads controls should be validated with the single color controls for stained cells

 

Compensation Beads Staining for Multi-Color Flow Cytometry Experiment