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BD LSRII-1 UV Laser Operation

NOTE: If the UV laser needs to be “on” for your experiment (Indo, Hoechst, AF350, CellTrace Calcein Blue AM, LIVE/DEAD Fixable Blue, etc.) please make sure to follow the steps explained below.

  1. 1. For the first time please make sure to be trained by the FCRC staff on this procedure. Training takes about 5 minutes and no advance scheduling is necessary. (business days, 9 am – 5 pm)
  2. 2. Double-click on the “Coherent Connection 4” icon on the desktop.
  3. 3. When the “Coherent Connection 4” interface fully opens, click the button “Start” and wait a few seconds until the UV laser’s power shows 19-20 mW emitting.
  4. 4. Wait 15 minutes for the data acquisition to make sure that the laser is completely warmed-up and stabilized.
  5. 5. Proceed on the item 5 from the “BD LSRII Maintenance Guidelines”.
  6. 6. After the data acquisition is finished make sure to switch off the UV laser by clicking “stop” button on its user interface then turn off the software.