The following procedure is for Plasmid DNA only. For BAC transgenic construct please contact GTF for special instructions.
To submit the DNA for pronuclear injection please prepare the following materials:
- 50ug of transgenic construct (insert plus vector) that has been digested with RE to remove vector.
- A picture of DNA gel to demonstrate the completion of RE digestion. Please indicate clearly on the picture which DNA fragment should be purified for injection.
- A map of your construct with your contact information.
- Please indicate on which mouse background the transgenic production should be.
GTF will purify the insert DNA for you.
GTF phone ext. 8650