Lysins: Novel Proteins to Detect and Eliminate Gram-Positive Bacterial Infections in Animals
The extraction and purification of genetic material from cells is often the first and most crucial step in life sciences research. Gram-positive bacteria, which contain thick cell walls, pose a challenge for efficient and effective nucleic acid extraction in this regard.
Dr. Vincent A. Fischetti and his research group have developed an effective enzyme-based nucleic acid extraction system. A group of enzymes isolated from viruses that infect specific bacteria (bacteriophages) was discovered as a potential antibacterial agent, and their mode of action is to compromise the integrity of the bacterial cell wall or membrane resulting in rapid lysis of the bacterial cell. Once the cell wall is removed, it is fairly straightforward to obtain the DNA or RNA for subsequent experiments, such as PCR, cloning, sequencing, and proteomics. The use of these enzymes as reagents for nucleic acid extraction shortens the time and increases the nucleic acid yield, which is greatly advantageous.
Several lysins available for licensing include:
|Bacterial Target||RU Reference Number||Lysin Name||Patent||References|
|Anthrax only||625||Ply G||US Pat. 7,402,309
US Pat. 8,580,553
|Schuch et al., Nature 2002, 418:884|
|651||Gamma & W||US Pat. 7,638,600||Schuch & Fischetti., J. Bacteriol. 2006, 188: 3037-3051|
|Anthrax only||755||Ply PH||US Pat 8,389,469||Yoong et al., J Bacteriol. 2006, 188 (7): 2711|
|S. pneumoniae||621||Pal||US Pat. 7,569,223|
|S. pneumoniae||960||CPL-1 dimer||WO/2013/052643|
|All Enterococci||654||Ply V12||US Pat. 7,582,291||Yoong et al., J Bacteriol. 2004, 186 (14):4808-4812|
|Group B Streptococcus||764||Ply GBS||US Pat 8,105,585||Cheng Q & Fischetti VA, Appl. Microb. Biotechnol. 2007, 74: 1284-1291.|
|All Staphylococci||885||Cly S||US Pat 8,840,900||Daniel et al., Antimicrobial Agents and
Chemotherapy 2010, 54: 1603-1612
|Clostridium difficile||1146||PlyCd||62/217,949||Wang et al. , Antimicrobiol Agents and Chemotherapy 2015, doi:10.1128/AAC.01357-15|
Highly specific for different bacterial strains.
- Development of new diagnostics for the detection of bacteria responsible for human and veterinary infections, environmental or food contamination.
- Development of novel veterinary therapeutics.
Stage of Development
Discovery –in vivo data available.
Vincent A Fischetti
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