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Found 35038 matches. Displaying 131-140
Kang JY, Mishanina TV, Landick R, Darst SA
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Mechanisms of Transcriptional Pausing in Bacteria

JOURNAL OF MOLECULAR BIOLOGY 2019 SEP 20; 431(20):4007-4029
Pausing by RNA polymerase (RNAP) during transcription regulates gene expression in all domains of life. In this review, we recap the history of transcriptional pausing discovery, summarize advances in our understanding of the underlying causes of pausing since then, and describe new insights into the pausing mechanisms and pause modulation by transcription factors gained from structural and biochemical experiments. The accumulated evidence to date suggests that upon encountering a pause signal in the nucleic-acid sequence being transcribed, RNAP rearranges into an elemental, catalytically inactive conformer unable to load NTP substrate. The conformation, and as a consequence lifetime, of an elemental paused RNAP is modulated by backtracking, nascent RNA structure, binding of transcription regulators, or a combination of these mechanisms. We conclude the review by outlining open questions and directions for future research in the field of transcriptional pausing. (C) 2019 Elsevier Ltd. All rights reserved.
Hernandez N
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Inherited IFNAR1 deficiency in otherwise healthy patients with adverse

JOURNAL OF EXPERIMENTAL MEDICINE 2019 SEP; 216(9):2057-2070
Vaccination against measles, mumps, and rubella (MMR) and yellow fever
Winnubst J, Bas E, Ferreira TA, Wu ZH, Economo MN, Edson P, Arthur BJ, Bruns C, Rokicki K, Schauder D, Olbris DJ, Murphy SD, Ackerman DG, Arshadi C, Baldwin P, Blake R, Elsayed A, Hasan M, Ramirez D, Dos Santos B, Weldon M, Zafar A, Dudman JT, Gerfen CR, Hantman AW, Korff W, Sternson SM, Spruston N, Svoboda K, Chandrashekar J
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Reconstruction of 1,000 Projection Neurons Reveals New Cell Types and Organization of Long-Range Connectivity in the Mouse Brain

CELL 2019 SEP 19; 179(1):268-281.e13
Neuronal cell types are the nodes of neural circuits that determine the flow of information within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides an essential descriptor of cell type and reveals how individual neurons route their output across the brain. Despite the importance of morphology, few projection neurons in the mouse brain have been reconstructed in their entirety. Here we present a robust and efficient platform for imaging and reconstructing complete neuronal morphologies, including axonal arbors that span substantial portions of the brain. We used this platform to reconstruct more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons constitute more than 85 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity.
Weitzenfeld P
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Antibodies targeting sialyl Lewis A mediate tumor clearance through

JOURNAL OF CLINICAL INVESTIGATION 2019 SEP 3; 129(9):3952-3962
Sialyl Lewis A (sLeA, also known as CA19-9), a tetrasaccharide
Yao NNY
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INTRACELLULAR ORGANIZATION Getting ready for DNA duplication

ELIFE 2019 SEP 27; 8(?):? Article e51291
The discovery of a biomolecular condensate involved in DNA replication
Petraitis V
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Ceftolozane-Tazobactam in the Treatment of Experimental Pseudomonas

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 2019 SEP; 63(9):? Article e00344-19
Ceftolozane-tazobactam (C/T) is a novel cephalosporin with in vitro
Kim GB, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra-Clarke M, Levy R, Watkins A, Park H, Abbasi Akhtar A, Linesch PW, Kobritz N, Chandra SS, Grausam K, Ayala-Sarmiento A, Molina J, Sedivakova K, Hoang K, Tsyporin J, Gareau DS, Filbin MG, Bannykh S, Santiskulvong C, Wang Y, Tang J, Suva ML, Chen B, Danielpour M, Breunig JJ
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Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements

CELL 2019 SEP 19; 179(1):251-267.e24
In situ transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mice but lack control over copy number, zygosity, and locus specificity. Here we establish mosaic analysis by dual recombinase-mediated cassette exchange (MADR), which permits stable labeling of mutant cells expressing transgenic elements from precisely defined chromosomal loci. We provide a toolkit of MADR elements for combination labeling, inducible and reversible transgene manipulation, VCre recombinase expression, and transgenesis of human cells. Further, we demonstrate the versatility of MADR by creating glioma models with mixed reporter-identified zygosity or with "personalized" driver mutations from pediatric glioma. MADR is extensible to thousands of existing mouse lines, providing a flexible platform to democratize the generation of somatic mosaic mice
Whicher JR
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Regulation of Eag1 gating by its intracellular domains

ELIFE 2019 SEP 6; 8(?):? Article e49188
Voltage-gated potassium channels (K(v)s) are gated by transmembrane
Wang TT
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Functional diversification of IgGs through Fc glycosylation

JOURNAL OF CLINICAL INVESTIGATION 2019 SEP 3; 129(9):3492-3498
IgG antibodies are secreted from B cells and bind to a variety of
Allocco AA
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Recessive Inheritance of Congenital Hydrocephalus With Other Structural

FRONTIERS IN CELLULAR NEUROSCIENCE 2019 SEP 26; 13(?):? Article 425
Background: ATP1A3 encodes the a 3 subunit of the Na+/K+ ATPase, a