Usage Rates for Fiscal Year 2023
EMRC USAGE (July 1, 2022-June 30, 2023)
| Category I (done by users) | Unit | RU users | Non-RU user |
| Use of High presure freezing | /run | $ 185.50 | $ 371.00 |
| Use of Freeze substitution | /day | $ 66.00 | $ 132.00 |
| Use of supporting device: CPD, Ion Sputter, Bio Wave, Glass knife maker, ultra microtome, Image analysis | /hour | $ 18.50 | $ 37.00 |
| Cryo-EM Preparation (Cryo Plunger, Cryo-Holder Evacuations, Heating plate, Ethane, LN2) | /hour | $ 18.50 | $ 37.00 |
| Scoping EM (Peak time -9am-6pm) | /hour | $ 53.00 | $ 106.00 |
| Scoping EM (Off Peak time – 6pm-9am) | /hour | $ 38.00 | $ 76.00 |
| Scoping (LM) | /hour | $ 38.00 | $ 76.00 |
| Category II (done by EMRC staff) | RU users | Non-RU user | |
| Initial consultation | /hour | FREE | $ 180.50 |
| Training | /hour | $ 86.50 | $ 173.00 |
| Scoping EM | /hour | $ 86.50 | $ 173.00 |
| Labor* | /hour | $ 86.50 | $ 173.00 |
| Use of High pressure freezer | /run | $ 185.50 | $ 371.00 |
| Use of Freeze substitution* | /day | $ 66.00 | $ 132.00 |
| Serial /Cryo sectioning | /hour | $ 124.00 | $ 248.00 |
| Miscellaneous supply | unit | $ 11.50 | $ 11.50 |
| Priority is placed for RU projects. Non-RU projects may be accepted depending on capacity and availability of device and/or EMRC staff. | |||
| Note: Examples of typical experiments: The following examples are provided as guidance for budget planning: | |||
| 1. Conventional TEM: discussion (1hr) + tissue process (~8hr) + cutting sections (~1hr/sample) + staining + scope time | |||
| 2. Conventional SEM: discussion (1hr) + tissue process (4-8hr) + coating + scope time | |||
| 3. Immuno EM: discussion (1hr) + Immuno-staining variable) + tissue process (~8hr) + cutting sections (1hr/sample) + staining + scope time | |||
| 4. Negative staining: discussion (1hr) + glow discharge (20 min) + negative staining + scope time | |||
| 5. Training of operation of EM: variable based on users past experience and own capabilities | |||
| *”Labor” includes but is not limited to the following items: | |||
| Processing tissue/cell for TEM or SEM | |||
| Immuno-EM | |||
| Negative staining | |||
| Cryo-EM Preparation | |||
| Staining | |||
| Use of supporting device: Critical Point Dryer, Ion Sputter, Bio Wave, Evaporator | |||
| Ultrathin sectioning | |||
| Computer image process and analysis | |||
| Methods and assay development | |||
The University reserves the right to modify usage rates.