Space, Motion, and Information Flow in the Bacterial Cell.
Event Details
- Type
- Center for Studies in Physics and Biology Seminars
- Speaker(s)
-
Sangjin Kim, Ph.D., Assistant Professor, University of Illinois Urbana-Champaign
- Speaker bio(s)
-
Bacterial cells organize a remarkable range of molecular activities within a small, crowded interior. My lab combines single-molecule imaging, quantitative analysis, genetics, and modeling to investigate how these activities unfold in space and time. I will first present our work on the coordination of transcription, translation, and RNA degradation. These studies reveal how translation activity and the cellular localization of RNA-degradation machinery constrain the fate of bacterial transcripts, defining when successive steps in the RNA life cycle are coupled or uncoupled. I will then introduce two ongoing studies that extend our investigation of the bacterial cell’s physical organization. Chromosome-tracking experiments reveal persistent subdiffusion and show how fluorescent labels influence the motion we measure. Measurements of cytoplasmic transport examine how different molecular probes exhibit distinct modes of motion within the same cellular environment. I will conclude with the questions and experimental approaches guiding our efforts to understand molecular motion and coordination in living bacterial cells.
- Open to
- Public
- Reception
- Refreshments, 3:30 p.m. - 4:00 p.m., Lower Level Greenberg Building (CRC)
- Phone
- (212) 327-8636
- Sponsor
-
Melanie Lee
(212) 327-8636
leem@rockefeller.edu