The Vosshall Lab freely shares all published reagents.
Fly stocks, DNA clones, vectors, and antibody reagents can be requested from the Vosshall Laboratory by sending an email to Leslie Vosshall.
Please note that unless you supply a FEDEX number, all reagent requests will be sent by regular mail. Please supply a complete shipping address and telephone contact number suitable for Fedex delivery (e.g. no PO boxes). The Vosshall Laboratory distributes all published reagents.
The very popular pCasperAUG-Gal4-X vector for cloning Drosophila enhancer/promoters in front of the Gal4 gene is available directly from Addgene. Addgene is also distributing a growing number of our historic Drosophila OR and GR plasmids as well as newer mosquito gene targeting vectors and mosquito cDNAs: Vosshall Lab Plasmid Collection at Addgene
Many of our published fly strains have been deposited in the Bloomington Drosophila Stock Center. This includes all OR-Gal4 transgenes, and Orco and Gr63a mutant reagents, as well as various tagged UAS-OR constructs. If you are having problems finding our stocks at Bloomington, please download their most current stock list and search for “Vosshall”.
Several of our published Aedes aegypti mutant strains can be ordered from the CDC MR4 resource in Atlanta, GA via BEI Resources. You must register with BEI Resources prior to ordering our live vector mosquitoes.
Mentoring Compact for Graduate Students and Postdocs in the Vosshall Laboratory (December 21, 2016)
Statement on Authorship Policies in the Vosshall Laboratory (February 23, 2015)
PRO TIP: How to give a chalk talk for a tenure-track position in the biomedical sciences (February 23, 2015)
How to rear beautiful and happy mosquitoes: Vosshall Lab Aedes aegypti Mosquito SOP Rearing Protocol (version December 12, 2014)
Genome-engineering with CRISPR-Cas9 in the mosquito Aedes aegypti.
Bench Manual for Precision Genome Editing in the Mosquito
Mosquito (Aedes aegypti) microinjection video filmed at Genetic Services Inc. by Ben Glass, Matt DeGennaro, and Conor McMeniman (February 24, 2011)
Whole Mount Fluorescent In Situ Hybridization for Drosophila Larval Brains
Enhanced Two-Color Fluorescent In Situ Hybridization for Low-Abundance Genes
Click here for the complete list of DOR proteins in Vosshall et al.,1999; Vosshall et al., 2000
Preparation of Tissue for Larval In Situ Hybridization (Frozen Sections)
Single Fly Genomic DNA Protocol
ABI Prism 310 Cycle Sequencing Protocol
ABI Prism 310 Cycle Sequencing Protocol – Economy Version
Complex cDNA Probes
Drosophila Embryo Microinjection
Egg Collecting Plates
Whole Mount Drosophila Embryo In Situ Hybridization with RNA probes
Whole Mount Fluorescent Antibody Staining of Drosophila Embryos
Expand High Fidelity PCR Reactions
Fixing Embryos for In Situ Hybridization
Making FIXATIVE for In Situ and Antibody Staining
Drosophila Genomic DNA Preparation
Genomic Southern Protocol
Hanahan Competent Cell Protocol
Screening Bacteriophage Lambda Libraries
Whole Mount Fluorescent Antibody Staining of Drosophila Larvae
Mass Dechorionation for Embryo Microinjection
Partial Restriction Digests
Pharmacia Spin Column Protocol
Plasmid Colony PCR
Stocker Fly Brain Immunofluorescence Protocol
DIG In Situ Hybridization Protocol(with detergent)
X-GAL STAINING [Frozen Cryostat Sections]
X-GAL STAINING [Adult Head Whole Mounts]
Annotation, localization, and clone information for all DOR genes (Excel file for download) (PDF)
Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation
Fly Head Sections Using Martin Heisenberg Fly Collars
Fly Food Protocol – 1x
Drosophila Larval Chemotaxis Assays
This material is based upon work supported by the National Science Foundation under Grant No. 0092693; the National Institutes of Health Grant number RO1 DC05036. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation