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Reagent Requests & Protocols

Robin Chemers Neustein Professor
Investigator, Howard Hughes Medical Institute

Reagent Requests

The Vosshall Lab freely shares all published reagents.

Fly stocks, DNA clones, vectors, and antibody reagents can be requested from the Vosshall Laboratory by sending an email to Leslie Vosshall.

Please note that unless you supply a FEDEX number, all reagent requests will be sent by regular mail. Please supply a complete shipping address and telephone contact number suitable for Fedex delivery (e.g. no PO boxes). The Vosshall Laboratory distributes all published reagents.

The very popular pCasperAUG-Gal4-X vector for cloning Drosophila enhancer/promoters in front of the Gal4 gene is available directly from Addgene. Addgene is also distributing a growing number of our historic Drosophila OR and GR plasmids as well as newer mosquito gene targeting vectors and mosquito cDNAs: Vosshall Lab Plasmid Collection at Addgene

Many of our published fly strains have been deposited in the Bloomington Drosophila Stock Center. This includes all OR-Gal4 transgenes, and Orco and Gr63a mutant reagents, as well as various tagged UAS-OR constructs. If you are having problems finding our stocks at Bloomington, please download their most current stock list and search for “Vosshall”.

Several of our published Aedes aegypti mutant strains can be ordered from the CDC MR4 resource in Atlanta, GA via BEI Resources. You must register with BEI Resources prior to ordering our live vector mosquitoes.

Protocols

Mentoring Compact for Graduate Students and Postdocs in the Vosshall Laboratory (December 21, 2016)

Statement on Authorship Policies in the Vosshall Laboratory (February 23, 2015)

PRO TIP: How to give a chalk talk for a tenure-track position in the biomedical sciences (February 23, 2015)

How to rear beautiful and happy mosquitoes: Vosshall Lab Aedes aegypti Mosquito SOP Rearing Protocol (version December 12, 2014)

Genome-engineering with CRISPR-Cas9 in the mosquito Aedes aegypti.

Bench Manual for Precision Genome Editing in the Mosquito

Mosquito (Aedes aegypti) microinjection video filmed at Genetic Services Inc. by Ben Glass, Matt DeGennaro, and Conor McMeniman (February 24, 2011)

Whole Mount Fluorescent In Situ Hybridization for Drosophila Larval Brains

Enhanced Two-Color Fluorescent In Situ Hybridization for Low-Abundance Genes

Click here for the complete list of DOR proteins in Vosshall et al.,1999; Vosshall et al., 2000

Preparation of Tissue for Larval In Situ Hybridization (Frozen Sections)

Single Fly Genomic DNA Protocol

ABI Prism 310 Cycle Sequencing Protocol

ABI Prism 310 Cycle Sequencing Protocol – Economy Version

Bacterial Transformation

Buck Buffer

Complex cDNA Probes

Drosophila Embryo Microinjection

Egg Collecting Plates

Whole Mount Drosophila Embryo In Situ Hybridization with RNA probes

Whole Mount Fluorescent Antibody Staining of Drosophila Embryos

Expand High Fidelity PCR Reactions

Fixing Embryos for In Situ Hybridization

Making FIXATIVE for In Situ and Antibody Staining

Drosophila Genomic DNA Preparation

Genomic Southern Protocol

Hanahan Competent Cell Protocol

Immunofluorescence

Screening Bacteriophage Lambda Libraries

Whole Mount Fluorescent Antibody Staining of Drosophila Larvae

Mass Dechorionation for Embryo Microinjection

Partial Restriction Digests

Pharmacia Spin Column Protocol

Plasmid Colony PCR

Stocker Fly Brain Immunofluorescence Protocol

DIG In Situ Hybridization Protocol(with detergent)

X-GAL STAINING [Frozen Cryostat Sections]

X-GAL STAINING [Adult Head Whole Mounts]

Annotation, localization, and clone information for all DOR genes (Excel file for download) (PDF)

Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation

Fly Head Sections Using Martin Heisenberg Fly Collars

Fly Food Protocol – 1x

Drosophila Larval Chemotaxis Assays

This material is based upon work supported by the National Science Foundation under Grant No. 0092693; the National Institutes of Health Grant number RO1 DC05036. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation





Contact Us

Laboratory of Neurogenetics and Behavior
Box No. 63
The Rockefeller University
1230 York Avenue
New York, NY 10065

Ms. Barbara Ghelardi
Lab Administrator

Leslie Vosshall