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Found 37769 matches. Displaying 8641-8650
Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, Hafner M, Borkhardt A, Sander C, Tuschl T
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RNA targets of wild-type and mutant FET family proteins
NATURE STRUCTURAL & MOLECULAR BIOLOGY 2011 DEC; 18(12):1428-1431
FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined global RNA targets for all human FET proteins and two ALS-causing human FUS mutants. FET members showed similar binding profiles, whereas FUS mutants showed a drastically altered binding pattern, consistent with changes in subcellular localization.
Stavropoulos N, Young MW
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insomniac and Cullin-3 Regulate Sleep and Wakefulness in Drosophila
NEURON 2011 DEC 22; 72(6):964-976
In a forward genetic screen in Drosophila, we have isolated insomniac, a mutant that severely reduces the duration and consolidation of sleep. Anatomically restricted genetic manipulations indicate that insomniac functions within neurons to regulate sleep. insomniac expression does not oscillate in a circadian manner, and conversely, the circadian clock is intact in insomniac mutants, suggesting that insomniac regulates sleep by pathways distinct from the circadian clock. The protein encoded by insomniac is a member of the BTB/POZ superfamily, which includes many proteins that function as adaptors for the Cullin-3 (Cul3) ubiquitin ligase complex. We show that Insomniac can physically associate with Cul3, and that reduction of Cul3 activity in neurons recapitulates the insomniac phenotype. The extensive evolutionary conservation of insomniac and Cul3 suggests that protein degradation pathways may have a general role in governing the sleep and wakefulness of animals.
Li LS, Rutlin M, Abraira VE, Cassidy C, Kus L, Gong SC, Jankowski MP, Luo WQ, Heintz N, Koerber HR, Woodbury CJ, Ginty DD
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The Functional Organization of Cutaneous Low-Threshold Mechanosensory Neurons
CELL 2011 DEC 23; 147(7):1615-1627
Innocuous touch of the skin is detected by distinct populations of neurons, the low-threshold mechano-receptors (LTMRs), which are classified as A beta-, A delta-, and C-LTMRs. Here, we report genetic labeling of LTMR subtypes and visualization of their relative patterns of axonal endings in hairy skin and the spinal cord. We found that each of the three major hair follicle types of trunk hairy skin (guard, awl/auchene, and zigzag hairs) is innervated by a unique and invariant combination of LTMRs; thus, each hair follicle type is a functionally distinct mechanosensory end organ. Moreover, the central projections of A beta-, A delta-, and C-LTMRs that innervate the same or adjacent hair follicles form narrow LTMR columns in the dorsal horn. These findings support a model of mechanosensation in which the activities of A beta-, A delta-, and C-LTMRs are integrated within dorsal horn LTMR columns and processed into outputs that underlie the perception of myriad touch sensations.
Kost RG, Lee LM, Yessis J, Coller BS, Henderson DK
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Assessing Research Participants' Perceptions of their Clinical Research Experiences
CTS-CLINICAL AND TRANSLATIONAL SCIENCE 2011 DEC; 4(6):403-413
Introduction: Participants perceptions of their research experiences provide valuable measures of ethical treatment, yet no validated instruments exist to measure these experiences. We conducted focus groups of research participants and professionals as the initial step in developing a validated instrument. Methods: Research participants enrolled in 12 focus groups, consisting of: (1) individuals with disorders undergoing interventions; (2) in natural history studies; or (3) healthy volunteers. Research professionals participated in six separate groups of: (1) institutional review board members, ethicists, and Research Subject Advocates; (2) research nurses/coordinators; or (3) investigators. Focus groups used standard methodologies. Results: Eighty-five participants and 29 professionals enrolled at eight academic centers. Altruism and personal relevance of the research were commonly identified motivators; financial compensation was less commonly mentioned. Participants were satisfied with informed consent processes but disappointed if not provided test results, or study outcomes. Positive relationships with research teams were valued highly. Research professionals were concerned about risks, undue influence, and informed consent. Conclusions: Participants join studies for varied, complex reasons, notably altruism and personal relevance. They value staff relationships, health gains, new knowledge, and compensation, and expect professionalism and good organization. On the basis of these insights, we propose specific actions to enhance participant recruitment, retention, and satisfaction. Clin Trans Sci 2011; Volume 4: 403-413
Wilczek C, Chitta R, Woo E, Shabanowitz J, Chait BT, Hunt DF, Shechter D
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Protein Arginine Methyltransferase Prmt5-Mep50 Methylates Histones H2A and H4 and the Histone Chaperone Nucleoplasmin in Xenopus laevis Eggs
JOURNAL OF BIOLOGICAL CHEMISTRY 2011 DEC 9; 286(49):42221-42231
Histone proteins carry information contained in post-translational modifications. Eukaryotic cells utilize this histone code to regulate the usage of the underlying DNA. In the maturing oocytes and eggs of the frog Xenopus laevis, histones are synthesized in bulk in preparation for deposition during the rapid early developmental cell cycles. During this key developmental time frame, embryonic pluripotent chromatin is established. In the egg, non-chromatin-bound histones are complexed with storage chaperone proteins, including nucleoplasmin. Here we describe the identification and characterization of a complex of the protein arginine methyltransferase 5 (Prmt5) and the methylosome protein 50 (Mep50) isolated from Xenopus eggs that specifically methylates predeposition histones H2A/H2A.X-F and H4 and the histone chaperone nucleoplasmin on a conserved motif(GRGXK). We demonstrate that nucleoplasmin (Npm), an exceedingly abundant maternally deposited protein, is a potent substrate for Prmt5-Mep50 and is monomethylated and symmetrically dimethylated at Arg-187. Furthermore, Npm modulates Prmt5-Mep50 activity directed toward histones, consistent with a regulatory role for Npm in vivo. We show that H2A and nucleoplasmin methylation appears late in oogenesis and is most abundant in the laid egg. We hypothesize that these very abundant arginine methylations are constrained to premid blastula transition events in the embryo and therefore may be involved in the global transcriptional repression found in this developmental time frame.
Warburg A, Faiman R, Shtern A, Silberbush A, Markman S, Cohen JE, Blaustein L
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Oviposition habitat selection by Anopheles gambiae in response to chemical cues by Notonecta maculata
JOURNAL OF VECTOR ECOLOGY 2011 DEC; 36(2):421-425
A number of mosquito species avoid predator-inhabited oviposition sites by detecting predator-released kairomones. In the laboratory, we found that when offered de-ionized water and de-ionized water conditioned with Notonecta maculata, gravid Anopheles gambiae females preferentially oviposited into the former. We then conducted further experiments using two chemical components found in Notonecta-conditioned water, chemically pure n-tricosane and/or n-heneicosane, that was previously shown to repel oviposition by Culiseta longiareolata. These hydrocarbons failed to deter oviposition by An. gambiae females. Thus, different mosquito species may rely on distinct chemical cues to avoid predators. Identification and chemical characterization of such kairomones could facilitate innovative, environmentally sound mosquito control.
Chatrchyan S, Khachatryan V, Sirunyan AM, Tumasyan A, Adam W, Bergauer T, Dragicevic M, Ero J, Fabjan C, Friedl M, Fruhwirth R, Ghete VM, Hammer J, Hansel S, Hoch M, Hormann N, Hrubec J, Jeitler M, Kiesenhofer W, Krammer M, Liko D, Mikulec I, Pernicka M, Rahbaran B, Rohringer H, Schofbeck R, Strauss J, Taurok A, Teischinger F, Trauner C, Wagner P, Waltenberger W, Walzel G, Widl E, Wulz CE, Mossolov V, Shumeiko N, Gonzalez JS, Bansal S, Benucci L, De Wolf EA, Janssen X, Luyckx S, Maes T, Mucibello L, Ochesanu S, Roland B, Rougny R, Selvaggi M, Van Haevermaet H, Van Mechelen P, Van Remortel N, Blekman F, Blyweert S, D'Hondt J, Suarez RG, Kalogeropoulos A, Maes M, Olbrechts A, Van Doninck W, Van Mulders P, Van Onsem GP, Villella I, Charaf O, Clerbaux B, De Lentdecker G, Dero V, Gay APR, Hammad GH, Hreus T, Marage PE, Raval A, Thomas L, Vander Marcken G, Vander Velde C, Vanlaer P, Adler V, Cimmino A, Costantini S, Grunewald M, Klein B, Lellouch J, Marinov A, Mccartin J, Ryckbosch D, Thyssen F, Tytgat M, Vanelderen L, Verwilligen P, Walsh S, Zaganidis N, Basegmez S, Bruno G, Caudron J, Ceard L, Gil EC, De Jeneret JD, Delaere C, Favart D, Forthomme L, Giammanco A, Gregoire G, Hollar J, Lemaitre V, Liao J, Militaru O, Nuttens C, Ovyn S, Pagano D, Pin A, Piotrzkowski K, Schul N, Beliy N, Caebergs T, Daubie E, Alves GA, Brito L, Damiao DD, Pol ME, Souza MHG, Alda WL, Carvalho W, Da Costa EM, Martins CD, De Souza SF, Figueiredo DM, Mundim L, Nogima H, Oguri V, Da Silva WLP, Santoro A, Do Amaral SMS, Sznajder A, Anjos TS, Bernardes CA, Dias FA, Tomei TRFP, Gregores EM, Lagana C, Marinho F, Mercadante PG, Novaes SF, Padula SS, Darmenov N, Genchev V, Iaydjiev P, Piperov S, Rodozov M, Stoykova S, Sultanov G, Tcholakov V, Trayanov R, Vutova M, Dimitrov A, Hadjiiska R, Karadzhinova A, Kozhuharov V, Litov L, Mateev M, Pavlov B, Petkov P, Bian JG, Chen GM, Chen HS, Jiang CH, Liang D, Liang S, Meng X, Tao J, Wang J, Wang J, Wang X, Wang Z, Xiao H, Xu M, Zang J, Zhang Z, Ban Y, Guo S, Guo Y, Li W, Mao Y, Qian SJ, Teng H, Zhu B, Zou W, Cabrera A, Moreno BG, Rios AAO, Oliveros AFO, Sanabria JC, Godinovic N, Lelas D, Lelas K, Plestina R, Polic D, Puljak I, Antunovic Z, Dzelalija M, Kovac M, Brigljevic V, Duric S, Kadija K, Luetic J, Morovic S, Attikis A, Galanti M, Mousa J, Nicolaou C, Ptochos F, Razis PA, Finger M, Finger M, Assran Y, Kamel AE, Khalil S, Mahmoud MA, Radi A, Hektor A, Kadastik M, Muntel M, Raidal M, Rebane L, Tiko A, Azzolini V, Eerola P, Fedi G, Voutilainen M, Czellar S, Harkonen J, Heikkinen A, Karimaki V, Kinnunen R, Kortelainen MJ, Lampen T, Lassila-Perini K, Lehti S, Linden T, Luukka P, Maenpaa T, Tuominen E, Tuominiemi J, Tuovinen E, Ungaro D, Wendland L, Banzuzi K, Karjalainen A, Korpela A, Tuuva T, Sillou D, Besancon M, Choudhury S, Dejardin M, Denegri D, Fabbro B, Faure JL, Ferri F, Ganjour S, Givernaud A, Gras P, de Monchenault GH, Jarry P, Locci E, Malcles J, Marionneau M, Millischer L, Rander J, Rosowsky A, Shreyber I, Titov M, Baffioni S, Beaudette F, Benhabib L, Bianchini L, Bluj M, Broutin C, Busson P, Charlot C, Dahms T, Dobrzynski L, Elgammal S, de Cassagnac RG, Haguenauer M, Mine P, Mironov C, Ochando C, Paganini P, Sabes D, Salerno R, Sirois Y, Thiebaux C, Veelken C, Zabi A, Agram JL, Andrea J, Bloch D, Bodin D, Brom JM, Cardaci M, Chabert EC, Collard C, Conte E, Drouhin F, Ferro C, Fontaine JC, Gele D, Goerlach U, Greder S, Juillot P, Karim M, Le Bihan AC, Mikami Y, Van Hove P, Fassi F, Mercier D, Baty C, Beauceron S, Beaupere N, Bedjidian M, Bondu O, Boudoul G, Boumediene D, Brun H, Chasserat J, Chierici R, Contardo D, Depasse P, El Mamouni H, Fay J, Gascon S, Ille B, Kurca T, Le Grand T, Lethuillier M, Mirabito L, Perries S, Sordini V, Tosi S, Tschudi Y, Verdier P, Viret S, Lomidze D, Anagnostou G, Beranek S, Edelhoff M, Feld L, Heracleous N, Hindrichs O, Jussen R, Klein K, Merz J, Mohr N, Ostapchuk A, Perieanu A, Raupach F, Sammet J, Schael S, Sprenger D, Weber H, Weber M, Wittmer B, Zhukov V, Ata M, Dietz-Laursonn E, Erdmann M, Hebbeker T, Heidemann C, Hinzmann A, Hoepfner K, Klimkovich T, Klingebiel D, Kreuzer P, Lanske D, Lingemann J, Magass C, Merschmeyer M, Meyer A, Papacz P, Pieta H, Reithler H, Schmitz SA, Sonnenschein L, Steggemann J, Teyssier D, Bontenackels M, Cherepanov V, Davids M, Flugge G, Geenen H, Giffels M, Ahmad WH, Hoehle F, Kargoll B, Kress T, Kuessel Y, Linn A, Nowack A, Perchalla L, Pooth O, Rennefeld J, Sauerland P, Stahl A, Tornier D, Zoeller MH, Martin MA, Behrenhoff W, Behrens U, Bergholz M, Bethani A, Borras K, Cakir A, Campbell A, Castro E, Dammann D, Eckerlin G, Eckstein D, Flossdorf A, Flucke G, Geiser A, Hauk J, Jung H, Kasemann M, Katsas P, Kleinwort C, Kluge H, Knutsson A, Kramer M, Krucker D, Kuznetsova E, Lange W, Lohmann W, Lutz B, Mankel R, Marienfeld M, Melzer-Pellmann IA, Meyer AB, Mnich J, Mussgiller A, Olzem J, Petrukhin A, Pitzl D, Raspereza A, Rosin M, Schmidt R, Schoerner-Sadenius T, Sen N, Spiridonov A, Stein M, Tomaszewska J, Walsh R, Wissing C, Autermann C, Blobel V, Bobrovskyi S, Draeger J, Enderle H, Gebbert U, Gorner M, Hermanns T, Kaschube K, Kaussen G, Kirschenmann H, Klanner R, Lange J, Mura B, Naumann-Emme S, Nowak F, Pietsch N, Sander C, Schettler H, Schleper P, Schlieckau E, Schroder M, Schum T, Stadie H, Steinbruck G, Thomsen J, Barth C, Bauer J, Berger J, Buege V, Chwalek T, De Boer W, Dierlamm A, Dirkes G, Feindt M, Gruschke J, Guthoff M, Hackstein C, Hartmann F, Heinrich M, Held H, Hoffmann KH, Honc S, Katkov I, Komaragiri JR, Kuhr T, Martschei D, Mueller S, Muller T, Niegel M, Oberst O, Oehler A, Ott J, Peiffer T, Quast G, Rabbertz K, Ratnikov F, Ratnikova N, Renz M, Rocker S, Saout C, Scheurer A, Schieferdecker P, Schilling FP, Schmanau M, Schott G, Simonis HJ, Stober FM, Troendle D, Wagner-Kuhr J, Weiler T, Zeise M, Ziebarth EB, Daskalakis G, Geralis T, Kesisoglou S, Kyriakis A, Loukas D, Manolakos I, Markou A, Markou C, Mavrommatis C, Ntomari E, Petrakou E, Gouskos L, Mertzimekis TJ, Panagiotou A, Saoulidou N, Stiliaris E, Evangelou I, Foudas C, Kokkas P, Manthos N, Papadopoulos I, Patras V, Triantis FA, Aranyi A, Bencze G, Boldizsar L, Hajdu C, Hidas P, Horvath D, Kapusi A, Krajczar K, Sikler F, Veres GI, Vesztergombi G, Beni N, Molnar J, Palinkas J, Szillasi Z, Veszpremi V, Karancsi J, Raics P, Trocsanyi ZL, Ujvari B, Beri SB, Bhatnagar V, Dhingra N, Gupta R, Jindal M, Kaur M, Kohli JM, Mehta MZ, Nishu N, Saini LK, Sharma A, Singh AP, Singh J, Singh SP, Ahuja S, Choudhary BC, Gupta P, Kumar A, Kumar A, Malhotra S, Naimuddin M, Ranjan K, Shivpuri RK, Banerjee S, Bhattacharya S, Dutta S, Gomber B, Jain S, Jain S, Khurana R, Sarkar S, Choudhury RK, Dutta D, Kailas S, Kumar V, Mehta P, Mohanty AK, Pant LM, Shukla P, Aziz T, Guchait M, Gurtu A, Maity M, Majumder D, Majumder G, Mazumdar K, Mohanty GB, Parida B, Saha A, Sudhakar K, Wickramage N, Banerjee S, Dugad S, Mondal NK, Arfaei H, Bakhshiansohi H, Etesami SM, Fahim A, Hashemi M, Hesari H, Jafari A, Khakzad M, Mohammadi A, Najafabadi MM, Mehdiabadi SP, Safarzadeh B, Zeinali M, Abbrescia M, Barbone L, Calabria C, Colaleo A, Creanza D, De Filippis N, De Palma M, Fiore L, Iaselli G, Lusito L, Maggi G, Maggi M, Manna N, Marangelli B, My S, Nuzzo S, Pacifico N, Pierro GA, Pompili A, Pugliese G, Romano F, Roselli G, Selvaggi G, Silvestris L, Trentadue R, Tupputi S, Zito G, Abbiendi G, Benvenuti AC, Bonacorsi D, Braibant-Giacomelli S, Brigliadori L, Capiluppi P, Castro A, Cavallo FR, Cuffiani M, Dallavalle GM, Fabbri F, Fanfani A, Fasanella D, Giacomelli P, Giunta M, Grandi C, Marcellini S, Masetti G, Meneghelli M, Montanari A, Navarria FL, Odorici F, Perrotta A, Primavera F, Rossi AM, Rovelli T, Siroli G, Travaglini R, Albergo S, Cappello G, Chiorboli M, Costa S, Potenza R, Tricomi A, Tuve C, Barbagli G, Ciulli V, Civinini C, D'Alessandro R, Focardi E, Frosali S, Gallo E, Gonzi S, Meschini M, Paoletti S, Sguazzoni G, Tropiano A, Benussi L, Bianco S, Colafranceschi S, Fabbri F, Piccolo D, Fabbricatore P, Musenich R, Benaglia A, De Guio F, Di Matteo L, Gennai S, Ghezzi A, Malvezzi S, Martelli A, Massironi A, Menasce D, Moroni L, Paganoni M, Pedrini D, Ragazzi S, Redaelli N, Sala S, de Fatis TT, Buontempo S, Montoya CAC, Cavallo N, De Cosa A, Dogangun O, Fabozzi F, Iorio AOM, Lista L, Merola M, Paolucci P, Azzi P, Bacchetta N, Bellan P, Bisello D, Branca A, Carlin R, Checchia P, Dorigo T, Dosselli U, Fanzago F, Gasparini F, Gasparini U, Gozzelino A, Lacaprara S, Lazzizzera I, Margoni M, Mazzucato M, Meneguzzo AT, Nespolo M, Perrozzi L, Pozzobon N, Ronchese P, Simonetto F, Torassa E, Tosi M, Vanini S, Zotto P, Zumerle G, Baesso P, Berzano U, Ratti SP, Riccardi C, Torre P, Vitulo P, Viviani C, Biasini M, Bilei GM, Caponeri B, Fano L, Lariccia P, Lucaroni A, Mantovani G, Menichelli M, Nappi A, Romeo F, Santocchia A, Taroni S, Valdata M, Azzurri P, Bagliesi G, Bernardini J, Boccali T, Broccolo G, Castaldi R, D'Agnolo RT, Dell'Orso R, Fiori F, Foa L, Giassi A, Kraan A, Ligabue F, Lomtadze T, Martini L, Messineo A, Palla F, Palmonari F, Segneri G, Serban AT, Spagnolo P, Tenchini R, Tonelli G, Venturi A, Verdini PG, Barone L, Cavallari F, Del Re D, Di Marco E, Diemoz M, Franci D, Grassi M, Longo E, Meridiani P, Nourbakhsh S, Organtini G, Pandolfi F, Paramatti R, Rahatlou S, Sigamani M, Amapane N, Arcidiacono R, Argiro S, Arneodo M, Biino C, Botta C, Cartiglia N, Castello R, Costa M, Demaria N, Graziano A, Mariotti C, Maselli S, Migliore E, Monaco V, Musich M, Obertino MM, Pastrone N, Pelliccioni M, Potenza A, Romero A, Ruspa M, Sacchi R, Sola V, Solano A, Staiano A, Pereira AV, Belforte S, Cossutti F, Della Ricca G, Gobbo B, Marone M, Montanino D, Penzo A, Heo SG, Nam SK, Chang S, Chung J, Kim DH, Kim GN, Kim JE, Kong DJ, Park H, Ro SR, Son DC, Son T, Kim JY, Kim ZJ, Song S, Jo HY, Choi S, Gyun D, Hong B, Jo M, Kim H, Kim TJ, Lee KS, Moon DH, Park SK, Seo E, Sim KS, Choi M, Kang S, Kim H, Kim JH, Park C, Park IC, Park S, Ryu G, Cho Y, Choi Y, Choi YK, Goh J, Kim MS, Lee B, Lee J, Lee S, Seo H, Yu I, Bilinskas MJ, Grigelionis I, Janulis M, Martisiute D, Petrov P, Polujanskas M, Sabonis T, Castilla-Valdez H, De La Cruz-Burelo E, Heredia-de La Cruz I, Lopez-Fernandez R, Villalba RM, Martinez-Ortega J, Sanchez-Hernandez A, Villasenor-Cendejas LM, Moreno SC, Valencia FV, Ibarguen HAS, Linares EC, Pineda AM, Reyes-Santos MA, Krofcheck D, Tam J, Butler PH, Doesburg R, Silverwood H, Ahmad M, Ahmed I, Asghar MI, Hoorani HR, Khalid S, Khan WA, Khurshid T, Qazi S, Shah MA, Shoaib M, Brona G, Cwiok M, Dominik W, Doroba K, Kalinowski A, Konecki M, Krolikowski J, Frueboes T, Gokieli R, Gorski M, Kazana M, Nawrocki K, Romanowska-Rybinska K, Szleper M, Wrochna G, Zalewski P, Almeida N, Bargassa P, David A, Faccioli P, Parracho PGF, Gallinaro M, Musella P, Nayak A, Pela J, Ribeiro PQ, Seixas J, Varela J, Afanasiev S, Belotelov I, Bunin P, Gavrilenko M, Golutvin I, Kamenev A, Karjavin V, Kozlov G, Lanev A, Moisenz P, Palichik V, Perelygin V, Shmatov S, Smirnov V, Volodko A, Zarubin A, Golovtsov V, Ivanov Y, Kim V, Levchenko P, Murzin V, Oreshkin V, Smirnov I, Sulimov V, Uvarov L, Vavilov S, Vorobyev A, Vorobyev A, Andreev Y, Dermenev A, Gninenko S, Golubev N, Kirsanov M, Krasnikov N, Matveev V, Pashenkov A, Toropin A, Troitsky S, Epshteyn V, Erofeeva M, Gavrilov V, Kaftanov V, Kossov M, Krokhotin A, Lychkovskaya N, Popov V, Safronov G, Semenov S, Stolin V, Vlasov E, Zhokin A, Belyaev A, Boos E, Dubinin M, Dudko L, Ershov A, Gribushin A, Kodolova O, Lokhtin I, Markina A, Obraztsov S, Perfilov M, Petrushanko S, Sarycheva L, Savrin V, Snigirev A, Andreev V, Azarkin M, Dremin I, Kirakosyan M, Leonidov A, Mesyats G, Rusakov SV, Vinogradov A, Azhgirey I, Bayshev I, Bitioukov S, Grishin V, Kachanov V, Konstantinov D, Korablev A, Krychkine V, Petrov V, Ryutin R, Sobol A, Tourtchanovitch L, Troshin S, Tyurin N, Uzunian A, Volkov A, Adzic P, Djordjevic M, Ekmedzic M, Krpic D, Milosevic J, Aguilar-Benitez M, Maestre JA, Arce P, Battilana C, Calvo E, Cerrada M, 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Measurement of the weak mixing angle with the Drell-Yan process in proton-proton collisions at the LHC
PHYSICAL REVIEW D 2011 DEC 8; 84(11):? Article 112002
A multivariate likelihood method to measure electroweak couplings with the Drell-Yan process at the LHC is presented. The process is described by the dilepton rapidity, invariant mass, and decay angle distributions. The decay angle ambiguity due to the unknown assignment of the scattered constituent quark and antiquark to the two protons in a collision is resolved statistically using correlations between the observables. The method is applied to a sample of dimuon events from proton-proton collisions at root s = 7 TeV collected by the CMS experiment at the LHC, corresponding to an integrated luminosity of 1.1 fb(-1). From the dominant u (u) over bar, d (d) over bar -> gamma*/Z -> mu(-)mu(+) process, the effective weak mixing angle parameter is measured to be sin(2)theta(eff) = 0.2287 +/- 0.0020 (stat.) +/- 0.0025 (syst.) This result is consistent with measurements from other processes, as expected within the standard model.
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Svyatkovskiy A, Marono MV, Yoo HD, Zablocki J, Zheng Y, Guragain S, Parashar N, Adair A, Boulahouache C, Ecklund KM, Geurts FJM, Padley BP, Redjimi R, Roberts J, Zabel J, Betchart B, Bodek A, Chung YS, Covarelli R, de Barbaro P, Demina R, Eshaq Y, Flacher H, Garcia-Bellido A, Goldenzweig P, Gotra Y, Han J, Harel A, Miner DC, Petrillo G, Sakumoto W, Vishnevskiy D, Zielinski M, Bhatti A, Ciesielski R, Demortier L, Goulianos K, Lungu G, Malik S, Mesropian C, Arora S, Atramentov O, Barker A, Contreras-Campana C, Contreras-Campana E, Duggan D, Gershtein Y, Gray R, Halkiadakis E, Hidas D, Hits D, Lath A, Panwalkar S, Park M, Patel R, Richards A, Rose K, Schnetzer S, Somalwar S, Stone R, Thomas S, Cerizza G, Hollingsworth M, Spanier S, Yang ZC, York A, Eusebi R, Flanagan W, Gilmore J, Gurrola A, Kamon T, Khotilovich V, Montalvo R, Osipenkov I, Pakhotin Y, Perloff A, Roe J, Safonov A, Sengupta S, Suarez I, Tatarinov A, Toback D, Akchurin N, Bardak C, Damgov J, Dudero PR, Jeong C, Kovitanggoon K, Lee SW, Libeiro T, Mane P, Roh Y, Sill A, Volobouev I, Wigmans R, Yazgan E, Appelt E, Brownson E, Engh D, Florez C, Gabella W, Issah M, Johns W, Johnston C, Kurt P, Maguire C, Melo A, Sheldon P, Snook B, Tuo S, Velkovska J, Arenton MW, Balazs M, Boutle S, Cox B, Francis B, Goadhouse S, Goodell J, Hirosky R, Ledovskoy A, Lin C, Neu C, Wood J, Yohay R, Gollapinni S, Harr R, Karchin PE, Don CKK, Lamichhane P, Mattson M, Milstene C, Sakharov A, Anderson M, Bachtis M, Belknap D, Bellinger JN, Carlsmith D, Cepeda M, Dasu S, Efron J, Friis E, Gray L, Grogg KS, Grothe M, Hall-Wilton R, Herndon M, Herve A, Klabbers P, Klukas J, Lanaro A, Lazaridis C, Leonard J, Loveless R, Mohapatra A, Ojalvo I, Parker W, Ross I, Savin A, Smith WH, Swanson J, Weinberg M
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Search for a Vectorlike Quark with Charge 2/3 in t plus Z Events from pp Collisions at root s 7 TeV
PHYSICAL REVIEW LETTERS 2011 DEC 29; 107(27):? Article 271802
A search for pair-produced heavy vectorlike charge-2/3 quarks, T, in pp collisions at a center-of-mass energy of 7 TeV, is performed with the CMS detector at the LHC. Events consistent with the flavor-changing-neutral-current decay of a T quark to a top quark and a Z boson are selected by requiring two leptons from the Z-boson decay, as well as an additional isolated charged lepton. In a data sample corresponding to an integrated luminosity of 1.14 fb(-1), the number of observed events is found to be consistent with the standard model background prediction. Assuming a branching fraction of 100% for the decay T -> tZ, a T quark with a mass less than 475 GeV/c(2) is excluded at the 95% confidence level.
Zaharatos GJ, Yu J, Pace C, Song Y, Vasan S, Ho DD, Huang YX
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HIV-1 and influenza antigens synthetically linked to IgG2a Fc elicit superior humoral responses compared to unmodified antigens in mice
VACCINE 2011 DEC 9; 30(1):42-50
Using murine IgG subclass molecules (IgG1 or IgG2a) synthetically fused to HIV-1 or influenza test antigens, we explored the potential for IgG Fc scaffolds to augment immunogenicity. Each antigen (Ag) was grafted onto a hinge-Fc scaffold containing all critical residues necessary for interaction with effector cells, thus retaining effector functions of the native IgG subclass. We hypothesized that the differential affinity of Fc gamma Rs for specific IgG subclasses would influence the magnitude of immune responses elicited by immunization with an Ag-IgG Fc fusion vaccine. We demonstrate here that the antigen-specific humoral response elicited by Ag-IgG2a fusion vaccines is at least tenfold greater than that elicited by native antigen, that this response is superior to that elicited by Ag-IgG1, and that the augmented antigen-specific humoral response elicited is Fc gamma receptor-dependent. (C) 2011 Elsevier Ltd. All rights reserved.
Hiller NL, Eutsey RA, Powell E, Earl JP, Janto B, Martin DP, Dawid S, Ahmed A, Longwell MJ, Dahlgren ME, Ezzo S, Tettelin H, Daugherty SC, Mitchell TJ, Hillman TA, Buchinsky FJ, Tomasz A, de Lencastre H, Sa-Leao R, Post JC, Hu FZ, Ehrlich GD
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Differences in Genotype and Virulence among Four Multidrug-Resistant Streptococcus pneumoniae Isolates Belonging to the PMEN1 Clone
PLOS ONE 2011 DEC 9; 6(12):? Article e28850
We report on the comparative genomics and characterization of the virulence phenotypes of four S. pneumoniae strains that belong to the multidrug resistant clone PMEN1 (Spain(23F) ST81). Strains SV35-T23 and SV36-T3 were recovered in 1996 from the nasopharynx of patients at an AIDS hospice in New York. Strain SV36-T3 expressed capsule type 3 which is unusual for this clone and represents the product of an in vivo capsular switch event. A third PMEN1 isolate - PN4595-T23 - was recovered in 1996 from the nasopharynx of a child attending day care in Portugal, and a fourth strain - ATCC700669 - was originally isolated from a patient with pneumococcal disease in Spain in 1984. We compared the genomes among four PMEN1 strains and 47 previously sequenced pneumococcal isolates for gene possession differences and allelic variations within core genes. In contrast to the 47 strains - representing a variety of clonal types - the four PMEN1 strains grouped closely together, demonstrating high genomic conservation within this lineage relative to the rest of the species. In the four PMEN1 strains allelic and gene possession differences were clustered into 18 genomic regions including the capsule, the blp bacteriocins, erythromycin resistance, the MM1-2008 prophage and multiple cell wall anchored proteins. In spite of their genomic similarity, the high resolution chinchilla model was able to detect variations in virulence properties of the PMEN1 strains highlighting how small genic or allelic variation can lead to significant changes in pathogenicity and making this set of strains ideal for the identification of novel virulence determinants.