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Found 37684 matches. Displaying 5921-5930
Conti F, Aragao WC, Prando C, Deswarte C, Hubeau M, Newburger PE, Casanova JL, Bustamante J, Condino-Neto A
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Phagocyte nicotinamide adenine dinucleotide phosphate oxidase activity in patients with inherited IFN-gamma R1 or IFN-gamma R2 deficiency
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 2015 MAY; 135(5):1393-1395
Kim J, Xiao H, Koh J, Wang YK, Bonanno JB, Thomas K, Babbitt PC, Brown S, Lee YS, Almo SC
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Determinants of the CmoB carboxymethyl transferase utilized for selective tRNA wobble modification
NUCLEIC ACIDS RESEARCH 2015 MAY 19; 43(9):4602-4613
Enzyme-mediated modifications at the wobble position of tRNAs are essential for the translation of the genetic code. We report the genetic, biochemical and structural characterization of CmoB, the enzyme that recognizes the unique metabolite carboxy-S-adenosine-L-methionine (Cx-SAM) and catalyzes a carboxymethyl transfer reaction resulting in formation of 5-oxyacetyluridine at the wobble position of tRNAs. CmoB is distinctive in that it is the only known member of the SAM-dependent methyltransferase (SDMT) superfamily that utilizes a naturally occurring SAM analog as the alkyl donor to fulfill a biologically meaningful function. Biochemical and genetic studies define the in vitro and in vivo selectivity for Cx-SAM as alkyl donor over the vastly more abundant SAM. Complementary high-resolution structures of the apo- and Cx-SAM bound CmoB reveal the determinants responsible for this remarkable discrimination. Together, these studies provide mechanistic insight into the enzymatic and non-enzymatic feature of this alkyl transfer reaction which affords the broadened specificity required for tRNAs to recognize multiple synonymous codons.
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A, Sharma M, Sheldon P, Snook B, Tuo S, Velkovska J, Arenton MW, Boutle S, Cox B, Francis B, Goodell J, Hirosky R, Ledovskoy A, Li H, Lin C, Neu C, Wood J, Clarke C, Harr R, Karchin PE, Don CKK, Lamichhane P, Sturdy J, Belknap DA, Carlsmith D, Cepeda M, Dasu S, Dodd L, Duric S, Friis E, Hall-Wilton R, Herndon M, Herve A, Klabbers P, Lanaro A, Lazaridis C, Levine A, Loveless R, Mohapatra A, Ojalvo I, Perry T, Pierro GA, Polese G, Ross I, Sarangi T, Savin A, Smith WH, Taylor D, Vuosalo C, Woods N
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Searches for supersymmetry using the M-T2 variable in hadronic events produced in pp collisions at 8 TeV
JOURNAL OF HIGH ENERGY PHYSICS 2015 MAY 15; ?(5):? Article 078
Searches for supersymmetry (SUSY) are performed using a sample of hadronic events produced in 8 TeV pp collisions at the CERN LHC. The searches are based on the M-T2 variable, which is a measure of the transverse momentum imbalance in an event. The data were collected with the CMS detector and correspond to an integrated luminosity of 19.5 fb(-1). Two related searches are performed. The first is an inclusive search based on signal regions defined by the value of the M-T2 variable, the hadronic energy in the event, the jet multiplicity, and the number of jets identified as originating from bottom quarks. The second is a search for a mass peak corresponding to a Higgs boson decaying to a bottom quark-antiquark pair, where the Higgs boson is produced as a decay product of a SUSY particle. For both searches, the principal backgrounds are evaluated with data control samples. No significant excess over the expected number of background events is observed, and exclusion limits on various SUSY models are derived.
Chang FY, Ternei MA, Calle PY, Brady SF
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Targeted Metagenomics: Finding Rare Tryptophan Dimer Natural Products in the Environment
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2015 MAY 13; 137(18):6044-6052
Natural product discovery from environmental genomes (metagenornics) has largely been limited to the screening, of existing,environmental DNA (eDNA) libraries: Here, we have coupled a chemical-biogeographic survey of chroinopyrrolic acid synthase (CPAS) gene diversity with targeted eDNA. library production to more efficiently access,rare tryptophan dinner (TD) biosynthetic gene clusters. A combination of :traditional and,synthetic biology-bed heterologous expression efforts using,eDNA derived gene clusters led to the production of hydroxysporine (1)and reductasporine (2), two bioactive TDs. As suggested by but phylogeneic analysis of CPAS genes, identified in our survey of dude eDNA. extracts, reductasporine (2) contains an unprecedented TD core structure: a pyrrolinium indolocarbazole core that is likely key to its unusual bioactivity profile. This work demonstrates the potential for the discovery Of structurally rare and biologically interesting,natural products using targeted metagenomics, Where environmental samples are prescreened to identify the most phylogenetically unique gene sequences and molecules associated with these genes are accessed through targeted metagenomic library construction and heterologous expression.
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A, Khalil S, Makouski M, Maravin Y, Saini LK, Shrestha S, Svintradze I, Gronberg J, Lange D, Rebassoo F, Wright D, Baden A, Calvert B, Eno SC, Gomez JA, Hadley NJ, Kellogg RG, Kolberg T, Lu Y, Marionneau M, Mignerey AC, Pedro K, Skuja A, Tonjes MB, Tonwar SC, Apyan A, Barbieri R, Bauer G, Busza W, Cali IA, Chan M, Di Matteo L, Dutta V, Ceballos GG, Goncharov M, Gulhan D, Klute M, Lai YS, Lee YJ, Levin A, Luckey PD, Ma T, Paus C, Ralph D, Roland C, Roland G, Stephans GSF, Stoeckli F, Sumorok K, Velicanu D, Veverka J, Wyslouch B, Yang M, Zanetti M, Zhukova V, Dahmes B, De Benedetti A, Gude A, Kao SC, Klapoetke K, Kubota Y, Mans J, Pastika N, Rusack R, Singovsky A, Tambe N, Turkewitz J, Acosta JG, Oliveros S, Avdeeva E, Bloom K, Bose S, Claes DR, Dominguez A, Suarez RG, Keller J, Knowlton D, Kravchenko I, Lazo-Flores J, Malik S, Meier F, Snow GR, Dolen J, Godshalk A, Iashvili I, Kharchilava A, Kumar A, Rappoccio S, Alverson G, Barberis E, Baumgartel D, Chasco M, Haley J, Massironi A, 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F, Xie W, Xu L, Yoo HD, Zablocki J, Zheng Y, Parashar N, Stupak J, Adair A, Akgun B, Ecklund KM, Geurts FJM, Li W, Michlin B, Padley BP, Redjimi R, Roberts J, Zabel J, Betchart B, Bodek A, Covarelli R, de Barbaro P, Demina R, Eshaq Y, Ferbel T, Garcia-Bellido A, Goldenzweig P, Han J, Harel A, Khukhunaishvili A, Miner DC, Petrillo G, Vishnevskiy D, Bhatti A, Ciesielski R, Demortier L, Goulianos K, Lungu G, Mesropian C, Arora S, Barker A, Chou JP, Contreras-Campana C, Contreras-Campana E, Duggan D, Ferencek D, Gershtein Y, Gray R, Halkiadakis E, Hidas D, Lath A, Panwalkar S, Park M, Patel R, Rekovic V, Salur S, Schnetzer S, Seitz C, Somalwar S, Stone R, Thomas S, Thomassen P, Walker M, Rose K, Spanier S, York A, Bouhali O, Eusebi R, Flanagan W, Gilmore J, Kamon T, Khotilovich V, Krutelyov V, Montalvo R, Osipenkov I, Pakhotin Y, Perloff A, Roe J, Rose A, Safonov A, Sakuma T, Suarez I, Tatarinov A, Akchurin N, Cowden C, Damgov J, Dragoiu C, Dudero PR, Faulkner J, Kovitanggoon K, Kunori S, 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Search for dark matter, extra dimensions, and unparticles in monojet events in proton-proton collisions at root s=8TeV
EUROPEAN PHYSICAL JOURNAL C 2015 MAY 29; 75(5):?
Results are presented from a search for particle dark matter (DM), extra dimensions, and unparticles using events containing a jet and an imbalance in transverse momentum. The data were collected by the CMS detector in proton-proton collisions at the LHC and correspond to an integrated luminosity of 19.7 fb(-1) at a centre-of-mass energy of 8 TeV. The number of observed events is found to be consistent with the standard model prediction. Limits are placed on the DM-nucleon scattering cross section as a function of the DM particle mass for spin-dependent and spinin-dependent interactions. Limits are also placed on the scale parameter MD in the Arkani-Hamed, Dimopoulos, and Dvali (ADD) model of large extra dimensions, and on the unparticle model parameter Lambda(U). The constraints on ADD models and unparticles are the most stringent limits in this channel and those on the DM-nucleon scattering cross section are an improvement over previous collider results.
Notti RQ, Bhattacharya S, Lilic M, Stebbins CE
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A common assembly module in injectisome and flagellar type III secretion sorting platforms
NATURE COMMUNICATIONS 2015 MAY; 6(?):? Article 7125
Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design.
Arvinen KMJ, Suarez-Farinas M, Savilahti E, Sampson HA, Berin MC
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Immune factors in breast milk related to infant milk allergy are independent of maternal atopy
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 2015 MAY; 135(5):1390-1393
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Measurement of jet multiplicity distributions in t(t)over-bar production in pp collisions at root s = 7 TeV (vol 74, 3014, 2014)
EUROPEAN PHYSICAL JOURNAL C 2015 MAY 19; 75(5):? Article 216
Ross JB, Huh D, Noble LB, Tavazoie SF
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Identification of molecular determinants of primary and metastatic tumour re-initiation in breast cancer
NATURE CELL BIOLOGY 2015 MAY; 17(5):651-U258
Through in vivo selection of multiple ER-negative human breast cancer populations for enhanced tumour-forming capacity, we have derived subpopulations that generate tumours more efficiently than their parental populations at low cell numbers. Tumorigenic-enriched subpopulations exhibited increased expression of LAMA4, FOXQ1 and NAP1L3-genes that are also expressed at greater levels by independently derived metastatic subpopulations. These genes promote metastatic efficiency. FOXQ1 promotes LAMA4 expression, and LAMA4 enhances clonal expansion following substratum detachment in vitro, tumour re-initiation in multiple organs, and disseminated metastatic cell proliferation and colonization. The promotion of cancer cell proliferation and tumour re-initiation by LAMA4 requires fil-integrin. Increased LAMA4 expression marks the transition of human pre-malignant breast lesions to malignant carcinomas, and tumoral LAMA4 overexpression predicts reduced relapse-free survival in ER-negative patients. Our findings reveal common features that govern primary and metastatic tumour re-initiation and identify a key molecular determinant of these processes.
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Combined Measurement of the Higgs Boson Mass in pp Collisions at root s=7 and 8 TeV with the ATLAS and CMS Experiments
PHYSICAL REVIEW LETTERS 2015 MAY 14; 114(19):? Article 191803
A measurement of the Higgs boson mass is presented based on the combined data samples of the ATLAS and CMS experiments at the CERN LHC in the H --> gamma gamma and H --> ZZ --> 4l decay channels. The results are obtained from a simultaneous fit to the reconstructed invariant mass peaks in the two channels and for the two experiments. The measured masses from the individual channels and the two experiments are found to be consistent among themselves. The combined measured mass of the Higgs boson is m(H) = 125.09 +/- 0.21 (stat) +/- 0.11 (syst) GeV.