March 28, 2008 Request your sorts by filling FCRC Sort Booking Form at http://www.rockefeller.edu/fcrc/form.php January 22, 2008 Alternate method of connecting to Central File Store in Flow Cytometry January 1, 2008 561 nm laser is now available on BD LSRII-2 new 16 color Advanced Analyzer September 22, 2006 BD LSR II and Analysis PC computers have been upgraded to DiVa 5.0.1 February 6, 2006 BD FACSAria - new 12 color Cell Sorter is now sorting at full capacity February 14, 2005 All the MACs at FCRC are running MAC OS X 10.3.5 and CellQuestPro 5.2 February 14, 2005 New login system at FCRC is based on the Central File Storage (CFS) login/password. Please contact the IT Help Desk at x8940. November 9, 2004 BD LSR II has been upgraded to twelve fluorescence detectors (488 nm/five, 355 nm/two, 407 nm/two and 633 nm/three).

The Flow Cytometry Resource Center (FCRC) provides University investigators with equipment and support for cell sorting (separation) from single cell suspension using fluorescence and for acquisition and analysis of flow cytometric data.

For analysis purposes, the FCRC is equipped with benchtop alignment-free multipurpose analyzers from BD Immunocytometry Systems (FACSCalibur, FACSort and BD LSR). The laser configurations on these instruments allow for analysis of cell samples stained with 488 nm, 633 nm and 325 nm (UV) excited dyes. A number of applications, including the multicolor analysis of cell phenotype, gene expression and cell cycle may be performed. After orientation training, the analyzers are available for use directly by the investigators.

• maintenance of the instruments,
  
  
• assistance with experimental design,
  
  
• advise on sample preparation,
  
  
• individualized instruction and training on the instruments, dependent upon the researcher's knowledge and applications requested,
  
  
• consultation on data analysis,
  
  
• experimental troubleshooting.
  
  

For cell sorting, the FCRC is equipped with a FACS Vantage SE cell sorter (BD Immunocytometry Systems). This instrument provides high-speed, high purity (up to 99.0%) multilaser cell sorting and is capable of sterile sorts of single cells directly into culture plates. The sorter is operated by FCRC staff only.

The mission of Flow Cytometry Resource Center is to offer researchers efficient and reliable flow cytometric services with the highest standards of quality control and productivity.