Flow Cytometry Resource Center
December 1, 2010
New Cell Sorter, BD FACSAria-3, equipped with 6-lasers (488, 640, 405, 561, 445, 355nm )/18-detectors is available for sorting
November 19, 2010
Optical configuration has changed for BD LSRII-1 and BD LSRII-2. Please CREATE NEW EXPERIMENTS and re-assign detectors
October 18, 2010
FCRC welcomes new staff member - Selam Tadesse
October 13, 2010
Six month FREE trial site license for FlowJo will expire on 4/10/2011. Please provide PTAEO to continue
September 20, 2010
To expand cell sorting capacity FCRC offers Saturday sorting for October-November-December 2010
August 23, 2010
Starting 8/23/10 please use CFS password for FCRC on-line booking
August 9, 2010
BD FACSAria-1 - had been upgraded with 561nm laser.
July 1, 2010
FCRC upgraded ImageStream100 to ImageStreamX with 488 nm, 407 nm, 561 nm and 658 nm lasers and ten fluorescent detectors
February 16, 2010
FCRC expanded Cell Sorting and ImageStream100 Schedule
January 15, 2010
FCRC welcomes two new staff members - Aliza Lloyd and Lily Xiao
July 20, 2009
ImageStream100's schedule is open for booking on Tuesdays, 11 am - 5 pm
March 26, 2009
FACSort retired after 15 years of service. Please use FACSCalibur-2 instead.
March 23, 2009
FCRC is relocated to new space - DWB205(Office), DWB207(Sort) and DWB211(Analysis)
March 9, 2009
All FCRC Analyzers have been moved to room DWB211. Be sure to activate your RU card for DWB211 access
The Flow Cytometry Resource Center (FCRC) provides University investigators with equipment and support for cell sorting (separation), acquisition, and analysis of flow cytometric data. All flow cytometry procedures require sample to be in the form of single cell suspension.
The Flow Cytometry Resource Center (FCRC) provides researchers with a wide variety of state-of-art multi-laser/multi-color flow cytometry sorters and analyzers, as well as an imaging flow cytometer. The FCRC staff provide maintenance of the instruments, assistance with experimental design and troubleshooting, advice on sample preparation, individualized instruction, and consultation on data analysis.
Cell sorting is performed by FCRC staff on three BD FACSAria cell sorters equipped with up to six-lasers (488, 561, 640, 355, 405, and 445 nm excitation wavelengths) and 18 fluorescence channel detection. All the cell sorters are able to perform high-speed (up to 20,000 events/sec), high-purity (up to 99.9%) sterile sorts into different types of devices (tubes, plates, slides, or custom-made devices).
The ImageStream-X combines the strength of flow cytometry and fluorescence microscopy in a single platform. It allows for high content assays on rare cells and quantification of biological phenomena with incredible accuracy. The ImageStream-X is able to simultaneously record up to 10-color images with the four-laser excitation (488, 561, 658, and 405 nm wavelengths). Data acquisition on ImageStream-X is performed by FCRC staff.
The FCRC is equipped with two advanced benchtop analyzers — BD LSRIIs equipped with up to five-lasers (488, 561, 640, 355, and 405 nm excitation wavelengths) and 16 fluorescence channels. The Center also has two basic analyzers — BD FACSCaliburs with two-lasers/four-channels. After completion of appropriate equipment training (Basic and Advanced Flow Cytometry Classes), researchers have full time access to and can operate the analyzers themselves.
The FCRC staff offers:
- Cell sorting on BD FACSAria
- Data acquisition on ImageStream-X
- Maintenance of all FCRC instruments
- Assistance and troubleshooting with experimental design
- Advise on sample preparation
- Individualized instruction and training on the benchtop flow cytometry analyzers, dependent upon the researcher's knowledge and applications requested
- Consultation on data analysis
- Instrumental and experimental troubleshooting
The mission of Flow Cytometry Resource Center is to offer researchers efficient and reliable flow cytometric services with the highest standards of quality control and productivity
