Flow Cytometry Resource Center
News and Features
September 26, 2011
The BD LSRII-2 upgraded with the 445-nm laser allows to analyze cells labeled CFP an other 445-nm excitable fluorochromes
September 4, 2011
ImageStream-X had been upgraded with the MultiMag Option. Check with FCRC Staff for benefits
August 20, 2011
FCRC installed new Basic Analyzer - Accuri C6 equipped with selectable lasers option and C-sampler
December 1, 2010
New Cell Sorter, BD FACSAria-3, equipped with 6-lasers (488, 640, 405, 561, 445, 355nm )/18-detectors is available for sorting
November 19, 2010
Optical configuration has changed for BD LSRII-1 and BD LSRII-2. Please CREATE NEW EXPERIMENTS and re-assign detectors
August 9, 2010
BD FACSAria-1 - had been upgraded with 561nm laser.
July 1, 2010
FCRC upgraded ImageStream100 to ImageStreamX with 488 nm, 407 nm, 561 nm and 658 nm lasers and ten fluorescent detectors
The Flow Cytometry Resource Center (FCRC) provides University investigators with equipment and support for cell sorting (separation), acquisition, and analysis of flow cytometric data. All flow cytometry procedures require sample to be in the form of single cell suspension.
The Flow Cytometry Resource Center (FCRC) provides researchers with a wide variety of state-of-art multi-laser/multi-color flow cytometry sorters and analyzers, as well as an imaging flow cytometer. The FCRC staff provides maintenance of the instruments, assistance with experimental design and troubleshooting, advice on sample preparation, individualized instruction, and consultation on data analysis.
Cell sorting is performed by FCRC staff on three BD FACSAria cell sorters equipped with up to six-lasers (488, 561, 640, 355, 405, and 445 nm excitation wavelengths) and 18 fluorescence channel detection. All the cell sorters are able to perform high-speed (up to 20,000 events/sec), high-purity (up to 99.9%) sterile sorts into different types of devices (tubes, plates, slides, or custom-made devices).
The ImageStream-X combines the strength of flow cytometry and fluorescence microscopy in a single platform. It allows for high content assays on rare cells and quantification of biological phenomena with incredible accuracy. The ImageStream-X is able to simultaneously record up to 10-color images with the four-laser excitation (488, 561, 658, and 405 nm wavelengths). Data acquisition on ImageStream-X is performed by FCRC staff.
The FCRC is equipped with two advanced benchtop analyzers — BD LSRIIs equipped with up to five-lasers (488, 561, 640, 355, and 405 nm excitation wavelengths) and 16 fluorescence channels. The Center also has two basic analyzers — BD FACSCaliburs and BD Accuri® C6 with two-lasers/four-channels. After completion of appropriate equipment training (Basic and Advanced Flow Cytometry Classes), researchers have full time access to and can operate the analyzers themselves.
The FCRC staff offers:
- Cell sorting on BD FACSAria
- Data acquisition on ImageStream-X
- Maintenance of all FCRC instruments
- Assistance and troubleshooting with experimental design
- Advise on sample preparation
- Individualized instruction and training on the benchtop flow cytometry analyzers, dependent upon the researcher's knowledge and applications requested
- Consultation on data analysis
- Instrumental and experimental troubleshooting
The mission of Flow Cytometry Resource Center is to offer researchers efficient and reliable flow cytometric services with the highest standards of quality control and productivity