The Rockefeller University - Flow Cytometry Resource Center (FCRC)

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Sunday, July 20, 2008




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Flow Cytometry Resource Center (FCRC)


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FCRC Sort Booking Form

Rockefeller University FCRC Online Sort Booking Form

Please fill every item on this form or it will NOT be submitted. If you answer "Other" to any question you will be contacted by FCRC for details.

*Name:

*E-mail:

*Lab:

*PI E-mail:

Date and Time

*Date requested: Second option:

*Start time requested: Flexibility:
(Ex: same day)

Duration suggested:

Cell Sorter Choice

FACSVantage SE
BD FACSAria

Regular pressure (70 psi)

Medium pressure (32 psi)

Not sure

Doesn't Matter
Not sure, need to discuss

*Have you done the same cell sort in the past at FCRC?

Yes No

Sample Information

*Will the samples be fixed before submission to FCRC?

Yes No

List type of sample:

*Eucaryotic cells: Yes No

*Description of cells: (source; ATCC#)

*Does sample contain any known infectious agent? (NOTE! If this sample has had contact with ANY human pathogen you MUST check yes. This includes during culture, preparation, experimental procedure, or from a patient sample)
Yes No

*Are the cells genetically modified?
Yes No

*Pre-sort Purification/Enrichment of the Sample?
Yes No

*Number of Samples:
*Total number of cells in experiment:	x 10⁶
*Final cell concentration of samples:	x 10⁶ to x 10⁶
*Fluorochromes: (Hold the option key and click to select multiple options on a PC, apple key and click for a Mac.)
Please enter any additional fluorochromes not in the list above:

FITC
GFP
Alexa488
CFDA-SE
YFP
PE
TRITC
PI
PE-TR
PE-Cy5
7AAD
RFP
PE-Cy5.5
PE-Cy7
DRAQ5
APC
Alexa633
Alexa647
To-Pro-3
Alexa700
APC-Cy7
APC-Alexa750
CFP
DAPI
Pacific Blue
DyeCycle Violet
Alexa 405
Aqua Live/Dead
AmCyan
Pacific Orange
mCherry
tdTomato
QDOT605
QDOT655
QDOT705
QDOT800
None