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High Throughput Screening Resource Center

The Rockefeller University HTS Center exists to aid in the search for small molecule or genetic modulators of biological functions. The center has a library of over 80,000 compounds and a collection of 94,000 human siRNAs spanning the entire genome. The center is configured for processing 96, 384 and 1536-well microtiter plates through the use of automated changeable tip dispensers, non-contact dispensers, syringe dispensers and pin tools. For assay technologies, the HTS Center has the capability to support cellular and biochemical assays using absorbance, fluorescent kinetics, fluorescence anisotropy, time-resolved fluorescence, time-resolved fluorescence resonance energy transfer, AlphaScreen, bioluminescence and cellular fluorescence imaging. Assay targets can include ion channels, receptors, enzymes, protein interactions, signaling pathways and cellular processes.

Objectives:

  1. To offer education, guidance and support for breakthroughs in biological science through the intelligent application of state-of-the-art high throughput screening technologies.
  2. To provide and continually improve a highly flexible facility which offers guidance in and access to technologies for assay development and miniaturization, screening and data analysis.
  3. To maintain high standards of communication, collaboration, efficiency and innovation.