The Rockefeller University Compound Collection is stored as 10 to 15 copies of 5 millimolar solutions in DMSO, heat-sealed and barcoded 384-well small-volume polypropylene microtiter plates. At least 8 archival copies are stored at -27C in REVCO freezers with emergency power and the two working copies are stored at -20C in a HighRes Biosolutions Nanocell system. The NanoCell system is comprised of a Liconic dry-storage random access deep freezer, a 5-axis robotic arm, an automated heat-sealer and de-sealer, a NanoServe plate carousel and barcode reader. Cellario scheduling software allows the system to flawlessly deliver library plates in short order. A "micro-dock" system allows for flexible integration of the screening instruments. The "working" copies of the library are used for a maximum of 10 freeze-thaw cycles. High-throughput cherry picking and serial dilution is accomplished with a Perkin-Elmer Janus automated pipette system capable of accurately selecting and arraying 1000 picks of 0.5 microliter of compound solution per day. Sample integrity of the library is periodically monitored by HPLC-MS of random samples.