Usage Rates for Fiscal Year 2013
(July 1, 2012 to June 30, 2013)
| EMRC Usage Rates for Y2013 (July 1, 2012 - June 30, 2013) | ||
|---|---|---|
| General | RU users ($) | Non-RU users ($) |
| Consultation | Free | 220/hour |
| Trainingδ | Free | 220/hour |
| Category I (done by users)* | ||
| Processing Tissue (manual) | Free | Free |
| Automated sample process | 70/run | 140/run |
| Immuno-EM | Free | Free |
| Negative Straining | Free | Free |
| Use of high pressure freezing unit | 185/run | 370/run |
| Use of freeze substitution unit | 91/run | 182/run |
| UA/Lead staining | Free | Free |
| Special techniques: critical point dry, ion spatter coat, glow discharge, rotary shadowing | 10/hour | 20/hour |
| Ultrathin sections | 10/hour | 20/hour |
| Scoping (TEM - SEM) | 42/hour | 84/hour |
| Miscellaneous supply | 10 | 20 |
| Category II (done by EMRC staff) | ||
| Processing tissue (up to 6 samples/run) | 60/hour | 120/hour |
| Immuno-EM (up to 6 samples/run)* | 62/hour | 124/hour |
| Use of high pressure freezing unit* | 185/run | 370/run |
| Use of freeze substitution unit (up to 5 days run)* | 91/run | 182/run |
| UA/Lead staining | 54/hour | 108/hour |
| Special techniques 1: Critical point dry, Ion spatter coat, Glow discharge. | 31/hour | 62/hour |
| Special techniques 2:Negative staining, Rotary shadowing, etc. | 54/hour | 108/hour |
| Ultrathin sections I (for samples prepared by EMRC staff) | 54/hour | 108/hour |
| Ultrathin sections II (for samples prepared by others) | 62/hour | 124/hour |
| Ultrathin sections III (serial section) | 90/hour | 180/hour |
| Cryo-ultrathin section | 90/hour | 180/hour |
| Scoping (TEM - SEM) | 60/hour | 120/hour |
| Miscellaneous labor | 55/hour | 110/hour |
| Miscellaneous supply | 10 | 20 |
δThe training fee is applicable only to the Non-Rockefeller users. For the users who wish to learn EM procedures, staff will train new users on procedures as they work together on real samples, with the overall project charged at Category II rates. Once the user has demonstrated competency, subsequent work is charged at Category I rates. The training will be customized based on a user's need so the total time required is variable.
* Immuno-EM as well as use of high pressure freezing unit or freeze substitution unit will be combined with Processing tissue for regular use.
Examples of typical experiments: The following examples are provided as guidance for time and budget planning:
- Conventional EM: discussion (1hr) + tissue process (~8hr) + cutting sections (~1hr/sample) + staining + scope time
- Immuno EM: discussion (1hr) + Immuno-staining (~10 hr) + tissue process (~8hr) + cutting sections (30-1hr/sample) + staining + scope time
- Negative staining: discussion (1hr) + glow discharge (20 min) + negative staining (20 min) + scope time
- Immuno EM and Negative staining: discussion (1hr) + glow discharge (10 min) + negative staining (20 min) + scope time
- Training of operation of EM: Approximately ~1hr of operation training.
