Heads of Laboratories
Laboratory of Protein and Nucleic Acid Chemistry
Ribosomes, molecular machines that catalyze protein synthesis in cells, must be able to accurately and completely translate messenger RNA into synthesized proteins that function in all domains of life. Dr. Klinge is interested in the structure and function of the proteins required for the assembly of the eukaryotic ribosome.
Ribosomes are giant molecular machines that are responsible for decoding the information contained in messenger RNA to synthesize proteins in all domains of life. Ribosome maturation, the process by which ribosomes are synthesized, is a very complex process, which involves approximately 200 factors in eukaryotes, of which most are essential for viability. These factors are involved in all stages of ribosome maturation, from transcription of ribosomal RNA in the nucleolus to export into the cytoplasm, where final stages of maturation occur. Our knowledge of the molecular machinery responsible for ribosome maturation is currently limited by a lack of structural information. Although work on the prokaryotic ribosome has advanced with structural studies of several important functional complexes, there had until recently been no atomic-level structural information about corresponding eukaryotic subunits. In contrast to their prokaryotic homologues, eukaryotic ribosomes are substantially larger, and this increase in size is accompanied by an overall increase in complexity.
As a postdoc, Dr. Klinge solved the 3.5-Ångström crystal structure of the Tetrahymena thermophila 60S ribosomal subunit, the first atomic description of any large eukaryotic ribosomal subunit. The structure illustrates the complex functional architecture of the eukaryotic 60S subunit, which comprises an intricate network of interactions between eukaryote-specific ribosomal protein features and RNA expansion segments. Eukaryote-specific elements cluster in two regions of the solvent-exposed side. In contrast to the prokaryotic 50S subunit, in which ribosomal proteins are isolated entities, the 60S ribosomal proteins are largely interconnected, giving rise to an intricate network of interactions. Eukaryote-specific proteins frequently fulfill key structural roles by interacting with RNA expansion segments. The structure further reveals the roles of eukaryotic ribosomal protein elements in the stabilization of the active site, since proteins in the vicinity of the catalytic center form an interconnected module.
At Rockefeller, Dr. Klinge works to solve the structures of macromolecular assemblies that catalyze key steps of ribosome maturation in eukaryotes. Structural information about these assemblies will represent a structural framework to understand the function of the complexes, and it will further provide a cornerstone for subsequent biochemical analysis.
Ultimately, Dr. Klinge hopes to reconstitute the initial stages of ribosome biogenesis in vitro. Such a system would facilitate the mechanistic study of different processing steps and allow for the isolation of specific intermediates, which cannot be obtained in vivo and may therefore provide novel approaches to study ribosome biogenesis.
B.A. in biochemistry, 2005
Ph.D. in biochemistry, 2009
University of Cambridge
Swiss Federal Institute of Technology in Zurich, 2009–2013
Assistant Professor, 2013–
The Rockefeller University
Human Frontier Science Program Career Development Award, 2014
Alfred P. Sloan Research Fellowship, 2014
Irma T. Hirschl/Monique Weill-Caulier Trust Research Award, 2014
Rita Allen Foundation Scholar, 2014
Holzer, S. et al. Crystal structure of the yeast ribosomal protein rpS3 in complex with its chaperone Yar1. J. Mol. Biol. 425, 4154–4160 (2013).
Klinge, S. et al. Atomic structures of the eukaryotic ribosome. Trends Biochem. Sci. 37, 189–198 (2012).
Klinge, S. et al. Crystal structure of the eukaryotic 60S ribosomal subunit in complex with initiation factor 6. Science 334, 941–948 (2011).
Klinge, S. et al. 3D architecture of DNA Pol α reveals the functional core of multi-subunit replicative polymerases. EMBO J. 28, 1978–1987 (2009).
Klinge, S. et al. An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis. Nat. Struct. Mol. Biol. 14, 875–877 (2007).
Dr. Klinge is a faculty member in the David Rockefeller Graduate Program, the Tri-Institutional M.D.-Ph.D. Program, and the Tri-Institutional Ph.D. Program in Chemical Biology.