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RU 621, 625, 651, 654, 755, 764, & 885

Reagents Package: Novel Enzymes for Efficient Nucleic Acid Extraction

Technology Summary

The extraction and purification of genetic material from cells is often the first and most crucial step in life sciences research.  Gram-positive bacteria, which contain thick cell walls, pose a challenge for efficient and effective nucleic acid extraction in this regard.

Dr. Vincent A. Fischetti and his research group have developed an effective enzyme-based nucleic acid extraction system. A group of enzymes isolated from viruses that infect specific bacteria (bacteriophages) was discovered as a potential antibacterial agent, and their mode of action is to compromise the integrity of the bacterial cell wall or membrane resulting in rapid lysis of the bacterial cell. Once the cell wall is removed, it is fairly straightforward to obtain the DNA or RNA for subsequent experiments, such as PCR, cloning, sequencing, and proteomics.  The use of these enzymes as reagents for nucleic acid extraction shortens the time and increases the nucleic acid yield, which is greatly advantageous.

Several lysins available for licensing include:

Bacterial Target RU Reference  Number Lysin Name Patent References
Anthrax only 625 Ply G US Pat. 7,402,309

US Pat. 8,580,553

Schuch et al., Nature 2002, 418:884
Anthrax

B. cereus

B. thuriengensis

651 Gamma & W US Pat.  7,638,600 Schuch & Fischetti., J. Bacteriol. 2006, 188: 3037-3051
Anthrax only 755 Ply PH US Pat 8,389,469 Yoong et al., J Bacteriol. 2006, 188 (7): 2711
S. pneumoniae 621 Pal US Pat. 7,569,223
S. pneumoniae 960 CPL-1 dimer WO/2013/052643
All Enterococci 654 Ply V12 US Pat. 7,582,291 Yoong et al., J Bacteriol. 2004, 186 (14):4808-4812
Group B Streptococcus 764 Ply GBS US Pat 8,105,585 Cheng Q & Fischetti VA, Appl. Microb. Biotechnol. 2007, 74: 1284-1291.
All Staphylococci 885 Cly S US Pat 8,840,900 Daniel et al., Antimicrobial Agents and

Chemotherapy 2010, 54: 1603-1612

Advantage

Highly specific for different bacterial strains.

Application

Development of research reagents for the effective lysis of specific bacterial cells.

Stage of Development

Discovery –in vivo data available.

Lead Inventor

Vincent A Fischetti

For Further Information