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Instruments

 

  • JANSi UVEX microscope for detecting protein crystals
  • Hampton Research cryo-tools for crystal minipulations in liquid nitrogen
  • Boyd cryo-tools and pucks for preparing samples for the use of the automounting robot at synchrotrons (BNL: X29, X6A, 12B, 12C, APS: NE-CAT 24-ID-C/E, GM/CA CAT 23, ALS: 5.0.1, 5.0.2, 5.0.3, 8.2.1 and 8.2.2)
  • Formulator by Formulatrix liquid handler for preparing crystallization reservoirs from stock solutions, in 96- or 24-well formats, into individual crystallization plates (regular and low profile) or deep well blocks for multiple setups
  • Phoenix by Art Robbins nano-volume liquid handler for filling reservoirs of 96-well trays and for placement of protein + reservoir nano volumes (200 nl – 2μl) as well as any additives, additional components or crystal seeds
  • SMZ18 by Nikon stereomicroscope for viewing trays and mounting crystals
  • Äkta Purifier by GE chromatographic system for protein purification
  • NGC Discover by Bio-Rad, multiwave chromatographic system for protein purification, room temperature
  • Phastsystem by Pharmacia (now GE) for electrophoresis analysis of microliter volumes
  • Crystal incubator, shakers, centrifuges
  • SEC-MALS Dawn Heleos-II by Wyatt Technologies for determining absolute molecular weights, oligomeric states, protein-protein interactions and conjugation

Contact

Rockefeller Research Building
Deena Oren, Ph.D.
Manager, Structural Biology Resource Center
The Rockefeller University
1230 York Avenue, Box 295
New York, NY 10065