The Structural Biology Resource Center (SBRC) located in RRB140 is an expert resource for protein expression and purification as well as all equipment needed for the determination of the three-dimensional structures of biological macromolecules via X-ray crystallography. The mission of the SBRC is to offer the expertise and tools that are the bread and butter of Structural Biology, to the benefit of research at The Rockefeller University.
UV fluorescence imaging of crystallization experiments is available in the Center. This technique allows the experimenter to determine whether very tiny crystals are in fact protein and not salt or other undesired components. This microscope is made by JanScientific and is capable of identifying Trp-containing protein crystals by excitation in the deep UV (280nm) and observing the emission at 350 nm. Also available is a stereomicroscope, Nikon SMZ18, for crystal tray observations and crystal mounting.
Robotic liquid handling is offered at the center, to facilitate in the task of setting up hundreds to thousands of crystallization conditions. The Formulator by Formulatrix prepares trays from stock solutions, reducing preparation time from days to minutes and the Phoenix by Art Robbins can dispense the reservoirs from trays made by Formulator (or obtained commercially) to individual trays and create the protein/precipitant drops using any 96-well crystallization tray.
For protein analysis the SBRC has a Phastsystem by Pharmacia (now GE) and an Äkta Purifier for purification of protein samples is available either as a hands-on instrument or as a fee for service by the SBRC staff. Separations may be based on affinity, sizing or ion-exchange.
The investigator performs all work independently after a short training session. The Center provides training and expert guidance for researchers undertaking crystallographic structure determination. The Center is also the RU liaison to the Advanced Photon Source and Brookhaven National Labs NSLS synchrotron X-ray PRT beam lines.
The SBRC offers:
- Assistance with experimental design in:
crystallography, protein expression or purification
- Individualized instruction and training on the instruments
- Consultation on structural information and data analysis
- Experimental troubleshooting
- Maintenance of the instruments
- HKL2000 support
- Synchrotron coordinator
The mission of the SBRC is to offer the most productive structural biology facility through high quality, reliability and open dialog to the benefit of research at The Rockefeller University.