Skip to main content
!
Phase III+: The University is open for expanded research operations; only authorized personnel will be admitted on campus. More info here.

Post Translational Modification

Analysis and identification of Post Translational Modification (or PTMs) is one of the strongest forces of mass spectromtery. However, it can also be one of the most tricky ones to analyses….. Identification rate (by LC-MS/MS) of Post Translational Modification phosphopeptides can be dramatically increased using enrichment strategies. In the PRC we currently utilize IMAC  and titanium dioxide – based phosphopeptide enrichment.  Both strategies are based on interaction of phosphorus-coordinated oxo groups with metal atoms (Fe and Ti). It is not always required or possible to apply enrichment strategies and the decision chart below helps to decide whether enrichment should be used or not.

Sorry. we are still working to add information to this page. Phosphorylation is a is

Phosphorylation is a posttranslational modification. Identification rate (by LC-MS/MS) of phosphopeptides can be dramatically increased using enrichment strategies. In the PRC we currently utilize IMAC  and titanium dioxide – based phosphopeptide enrichment.  Both strategies are based on interaction of phosphorus-coordinated oxo groups with metal atoms (Fe and Ti). It is not always required or possible to apply enrichment strategies and the decision chart below helps to decide whether enrichment should be used or not.

Typical workflow for PTM analysis by LC-MS/MS

Typical workflow for PTM analysis by LC-MS/MS

 

A example of an antibody based enrichment strategy is the the strategy used for the analysis of ubiquitin modified proteins/peptides.
Ubiquitin modification analysis by LCMS

Ubiquitin modification analysis by LCMS

 

Enrichment vs no enrichment

Enrichment allows to focus on what matters.