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Dimethyl labeling based quantitation

Stable isotope labeling using reductive dimethylation is cost-effective, simple quantitation method able to compete at any level with the other chemical labeling strategies. Simple reagent, formaldehyde, is used to globally label the N-terminus of the peptide and epsilon-amino group of Lysines through reductive amination. This labeling strategy produces peaks differing by 28 Da for each derivatized site relative to its nonderivatized counterpart and 4 Da units for each derivatized isotopic pair. Up to three different experiments can be simultaneously compared using this technique.

The complete experimental procedure for dimethyl labeling can be found here.


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