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Microarrays for Gene Expression, SNP Genotyping and CNV Analysis

Affymetrix Expression Arrays

Our Affymetrix expression array service can be used in two ways:

  • Full service: Users only need to provide us total RNA samples. We will check sample quantity and quality with Agilent BioAnalyzer, amplify and label the samples, set up hybridization, process and scan the arrays, and transfer data to users.Sample submission:
    Minimum 0.5 µg total RNA in 10 µl volume for 3’IVT arrays
    Minimum 1.0 µg total RNA in 10 µl volume for whole transcriptome arrays
  • Array hybridization and processing only: Users provide us biotin-labeled, fragmented cRNA samples. We will set up hybridization, process and scan the arrays, and transfer data to users.Sample submission:
    20 µg fragmented cRNA in maximum 40 µl volume

Affymetrix Whole Genome Arrays for SNP Genotyping and CNV Analysis

Affymetrix Genome-wide Human SNP Arrays (10K, 100K, 500K, SNP 5.0, and SNP 6.0)

  • Full service: Users only need to provide us genomic DNA samples with an agarose gel picture to show DNA integrity.  We will amplify and label the samples, set up hybridization, process and scan the arrays, and transfer data to users.Sample submission: Minimum 10 µl of genomic DNA at 50 ng/µl in low-EDTA TE buffer (10 mM Tris, 0.1 mM EDTA, pH 8.0).

Samples must not be significantly degraded. Samples should be free of PCR inhibitors. We recommend that users perform regular PCR to ensure that the genomics DNA samples amplify well.

Illumina Arrays

Illumina has discontinued all gene expression array products, as well as SNP arrays based on GoldenGate assays (including custom SNP and Mouse SNP arrays).  As a result, we no longer provide Illumina microarray services.