BD Accuri® C6
Note: BD Accuri C6 is retired in FY23 and is not available at FCRC
The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors.
The default configuration of the BD Accuri® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC.
The Selectable Lasers Module allows operation of the system in two additional configurations (2-Blue 2-Red, and 4-Blue) and significantly expands the fluorochrome combinations that can be analyzed.
BD Accuri® C6 is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. Data is digitally collected over a 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed.
A unique low-pressure pumping system drives the fluidics. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration of over 5 x 106 cells per mL. In addition, the system derives sample volume and can calculate absolute counts or sample concentration per microliter.
The non-pressurized system of BD Accuri C6 supports any brand of sample tubes – 12 x 75 mm or smaller (including microcentrifuge tubes), made of polypropylene or polystyrene.
BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from 48- and 96-well plates and deep-well plates. It is also supplied with a 24-tube rack for the walk-away acquisition of standard 12 x 75-mm tubes.
- BD AccuriC6 Maintenance Guidelines
- BD Accuri® C6 Filters and Fluorochromes list
- BD Accuri® C6 Optical Configuration Tables
- BD Accuri®C6 C Comp Calculator Spreadsheet
- BD Accuri®C6 Suggested Comp Values
- BD Accuri® C6 Absolute Counting
- BD Accuri® C6 Threshold Analysis Small Particles
- BD Accuri® C6 to FlowJo
- BD Accuri® C6 to FCS Express