Raphael Turcotte, Ph.D.
, Postdoctoral Research Assistant, University of Oxford
Optical microscopy has become an essential tool for neuroscientists. The primary reason for this success is that light enables the interrogation of living tissue in its physiological context at the length-scale of neurons and their subcellular components. Nevertheless, the quality of optical images is depth-dependent, because biological samples induce aberrations and scatter light, which ultimately limits imaging depth. In this talk, Turcotte will discuss how wavefront control (WC), an approach enabling the sculpting of light, can alleviate these issues for brain imaging in live animals for several imaging modalities. First, Turcotte will show that WC can be implemented for two-photon fluorescence imaging to improve the quality of structural imaging and the accuracy of functional measurements. Then, Turcotte will illustrate how WC is essential for achieving super-resolution imaging (structured illumination microscopy) in the living brain. Finally, Turcotte will examine how WC plays an essential role in minimally invasive micro-endoscopy with multimode optical fibers and thus enables subcellular imaging centimeters into the brain in vivo.