The Lancefield Collection of Streptococcus Strains

C-terminal-Linked Surface Proteins from Gram-Positive Bacteria
 NAME / GENEFUNCTION / NAME ORGANISM LPXTG REFERENCEACCESS#
1. emm6M protein S. pyogenesLPSTG(24)M11338
2. emm5M protein S. pyogenesLPSTG(40)M20374
3. emm12M proteinS. pyogenesLPSTG(52)U02342
4. emm24M proteinS. pyogenesLPSTG(41)M19031
5. emm49M proteinS. pyogenesLPSTG(19)M23689
6. emm57M proteinS. pyogenesLPSTG(37)X60959
7. emm2M proteinS. pyogenesLPSTG(3)X61276
8. emm3M proteinS. pyogenesLPSTG(31)Z21845
9. ARP2IgA binding proteinS. pyogenesLPSTG(3)X61276
10. ARP4IgA binding proteinS. pyogenesLPSTG(14)X15198
11. Mrp4IgG/fibrinogen bindingS. pyogenesLPSTG(44)M87831
12. FcRA Fc binding protein S. pyogenesLPSTG(20)M22532
13.Prot HHuman IgG Fc binding S. pyogenesLPSTG(17)M29398
14. SCPC5a peptidaseS. pyogenesLPTTN(7)J05229
15. T6Protease resistant proteinS. pyogenesLPSTG(54)M32978
16. sof22Serum opacity factorS. pyogenesLPASG(50)U02290
17. SfbFibronectin bindingS. pyogenesLPATG(61)X67947
18. ZAGBinds ?2 M, Alb, IgGS. zooepidemicusLPTTG(27)U02290
19. PrtFFibronectin bindingS. pyogenesLPATG(55)L10919
20. PAMPlasmin bindingS. pyogenesLPSTG(1)Z22219
21. Prot L Light chain bindingP. magnus LPKAG(30)M86697
22. PABHSA bindingP. magnusLPEAG(11)X77864
23. bacIgA binding proteinGr. B strepLPYTG(21)X58470
24. bcaalpha C antigenGr. B strepLPATG(39)M97256
25. fnbAFibronectin bindingS. dysgalactiaeLPQTG(36)Z22150
26. fnbBFibronectin bindingS. dysgalactiaeLPAAG(36)Z22151
27. FnzFibronectin bindingS. equiLPQTS(42)Y17116
28. SeMM-likeS. equiLPSTG(62)U73162
29. SzPSeM-likeS. equiLPQTS(62)U73163
30. Prot GIgG binding protein Gr. G strepLPTTG(46)X06173
31. EmmG1M proteinGr. G StrepLPSTG(9)M95774
32. DG12Albumin binding proteinGr. G strepLPSTG(57)M95520
33. GfbAFibronectin bindingGr. G. strepLPATG(33)U31115
34. MRPSurface protein*S. suisLPNTG(58)X64450
35. PAcSurface protein S. mutansLPNTG(45)X14490
36. spaP Surface protein S. mutansLPNTG(32)X17390
37. spaASurface proteinS. sobrinusLPATG(63)D90354
38. wapAWall-associated protein AS. mutansLPSTG(13)M19347
39. fruAFructosidaseS. mutansLPDTG(5)L03358
40. Sec10Surface proteinE. fecalisLPQTG(29)M64978
41. Asc10Surface proteinE. fecalisLPKTG(29)M64978
42. asa1Aggregation substanceE. fecalisLPQTG(16)X17214
43. Prot AIgG binding protein S. aureusLPETG(18)X00342
44. FnBPbFibronectin binding S. aureusLPETG(56)J04151
45.FnBPaFibronectin bindingS. aureusLPETG(28)X62992
46. cnaCollagen binding proteinS. aureusLPKTG(49)M81736
47. clfAClumping factorS. aureusLPDTG(38)Z18852
48. EDINEpidermal cell inhibitorS. aureusLPRGT(25)NA**
49. prtMCell wall proteaseL. paracaseiLPKTA(23)M83946
50. wg2Cell wall protease S. cremorisLPKTG(34)M24767
51. inlAInternalization proteinL. monocytogenesLPTTG(15)M67471
52. FimbriaeType 1 fimbriaeA. viscosisLPLTG(67)M32067
53. FimbriaeType 2 fimbriaeA. naeslundiiLPLTG(66)M21976
54. FimAType 2 fimbriaeA. naeslundiiLPLTG(68)AF019629
55. Hyal1HyaluronidaseS. pneumoniaeLPQTG(2)L20670
56. nanANeuraminidaseS. pneumoniaeLPETG(6)X72967
57.glnAGlutamine synthetaseGroup B strepLPATL(59)U61271
58. Protein F2Fibronectin bindingS. pyogenesLPATG(26)U31980
59. FbeFibrinogen bindingS. epidermidisLPDTG(43)Y17116
60. InlC2Internalin-likeL. monocytogenesLPTAG(12)U77368
61. InlDInternalin-likeL. monocytogenesLPTAG(12)U77368
62. InlEInternalin-likeL. monocytogenesLP I TG(12)U77368
63.InlFInternalin-likeL. monocytogenesLPKTG(12)U77367
64.SfBP1Fibronectin bindingS. pyogenesLPXTG(53)AF071083
65. dexDextranaseS. sobrinusLPKTG(64)M96978
66. pad1PheromoneE. fecalisLPHTG(65)X62658
67. FAIFibrinogen/albumin/IgGGroup C streptococcusLPSTG(60)NA
68. SfbIIFibronectin bindingS. pyogenesLPASG(35)X83303
69. spy0130 Minor pilin proteinS. pyogenes LPSTG (70) 
70. spy0128 Major pilin protein S. pyogenes EVPTG (70) 

Surface proteins attached by other mechanisms:
1. ftfFructosyl transferaseS. salivarius(51)
2. pspASurface proteinS. pneumoniae(69)
3. aasAdhesin/autolyticS. saprophiticus(22)
4. FbpAFibrinogen bindingS. aureus(8)
5. FBP54Fibronectin/fibrinogen bindS. pyogenes(10)
6. FibFibronectin bindingS. aureus(4)
7. SENSurface enolase S. pyogenes(48)
8. SDHSurface dehydrogenaseS. pyogenes(47)
* Surface proteins - proteins that have been identified to have a C-terminal anchor motif but the function is unknown.
** NA - Not available

Reference List
1.Berge, A. and U. Sjobring. 1993. PAM, a novel plasminogen-binding protein from Streptococcus pyogenenes. J.Biol.Chem. 268(34):25417-25424.
2.Berry, A.M., R.A. Lock, S.M. Thomas, D.P. Rajan, D. Hansman, and J.C. Paton. 1994. Cloning and nucleotide sequence of the Streptococcus pneumoniae hyaluronidase gene and purification of the enzyme from recombinant Escherichia coli. Infect.Immun. 62(3):1101-1108.
3.Bessen, D.E. and V.A. Fischetti. 1992. Nucleotide sequences of two adjacent M and M-like protein genes of group A streptococci: different RNA transcript levels and identification of a unique IgA-binding protein. Infect.Immun.60:124-135.
4.Boden, M.K. and J.-I. Flock. 1994. Cloning and characterization of a gene for a 19kDa fibrinogen-binding protein from staphylococcus aureus. Molecular Microbiology 12:599-606.
5.Burne, R.A. and J.E.C. Penders. 1993. Characterization of the Streptococcus mutans GS-5 fruA gene encoding Exo-beta-D-fructosidase. Infect.Immun. 60(11):4621-4632.
6.Camara, M., G.J. Boulnois, P.W. Andrew, and T.J. Mitchell. 1994. A neuraminidase from Streptococcus pneumoniae has the features of a surface protein. Infect.Immun. 62(9):3688-3695.
7.Chen, C.C. and P.P. Cleary. 1990. Complete nucleotide sequence of the streptococcal C5a peptidase gene of Streptococcus pyogenes. J.Biol.Chem. 265:3161-3167.
8.Cheung, A.I., S.J. Projan, R.E. Edelstein, and V.A. Fischetti. 1995. Cloning, expression, and nucleotide sequence of a staphylococcus aureus (fbpA)encoding a fibrinogen-binding protein. Infection and Immunity63:1914-1920.
9.Collins, C.M., A. Kimura, and A.L. Bisno. 1992. Group G streptococcal M protein exhibits structural features analogous to those of class I M protein of group A streptococci. Infect.Immun. 60:3689-3696.
10.Courtney, H.S., Y. Li, J.B. Dale, and D.L. Hasty. 1994. Cloning, sequencing, and expression of a fibronectin/fibrinogen-binding protein from group A streptococci. Infect.Immun. 62(9):3937-3946.
11.de Chateau, M. and L. Bjorck. 1994. Protein PAB, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling. J.Biol.Chem. 269(16):12147-12151.
12.Dramsi, S., P. Dehoux, M. Lebrun, P.L. Goossens, and P. Cossart. 1998. Identification of four new members of the internalin multigene family of Listeria monocytogenes EGD. Infection and Immunity 65:1615-1625.
13.Ferretti, J.J., R.R.B. Russell, and M.L. Dao. 1989. Sequence analysis of the wall-associated protein precursor of Streptococcus mutans antigen A. Molec.Microbiol. 3:469-478.
14.Frithz, E., L.-O. Heden, and G. Lindahl. 1989. Extensive sequence homology between IgA receptor and M protein in Streptococcus pyogenes. Molec.Microbiol. 3:1111-1119.
15.Gaillard, J.L., P. Berche, C. Frehel, E. Gouin, and P. Cossart. 1991. Entry of L. monocytogenes into Cells Is Mediated by a Repeat Protein Analgous to Surface Antigens from Gram-Positive, Extracellular Pathogens. Cell 65:1127-1141.
16.Galli, D., F. Lottspeich, and R. Wirth. 1990. Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1. Molec.Microbiol. 4:895-904.
17.Gomi, H., T. Hozumi, S. Hattori, C. Tagawa, F. Kishimoto, and L. Bjorck. 1990. The gene sequence and some properties of protein H. J.Immunol. 144:4046-4052.
18.Guss, B., M. Uhlen, B. Nilsson, M. Lindberg, J. Sjoquist, and J. Sjodahl. 1984. Region X, the cell-wall-attachment part of staphylococcal protein A. Eur.J.Biochem. 138:413-420.
19.Haanes, E.J. and P.P. Cleary. 1989. Identification of a divergent M protein gene and an M protein related gene family in serotype 49 Streptococcus pyogenes. J.Bacteriol. 171:6397-6408.
20.Heath, D.G. and P.P. Cleary. 1989. Fc-receptor and M protein genes of group A streptococci are products of gene duplication. Proc.Natl.Acad.Sci.U.S.A. 86:4741-4745.
21.Heden, L.-O., E. Frithz, and G. Lindahl. 1991. Molecular characterization of an IgA receptor from Group B Streptococci: sequence of the gene, identification of a proline-rich region with unique structure and isolation of N-terminal fragments with IgA-binding capacity. Eur.J.Immunol. 21:1481-1490.
22.Hell, W., H.-G.W. Meyer, and S.G. Gatermann. 1998. Cloning of aas, a gene encoding a staphylococcus saprophyticus surface protein with adhesive and autolytic properties. Molec.Microbiol. 29:871-881.
23.Holck, A. and H. Naes. 1992. Cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from Lactobaccillus paracasei subsp. paracasei NCDO 151. J.Gen.Microbiol. 138:1353-1364.
24.Hollingshead, S.K., V.A. Fischetti, and J.R. Scott. 1986. Complete nucleotide sequence of type 6 M protein of the group A streptococcus: repetitive structure and membrane anchor. J.Biol.Chem. 261:1677-1686.
25.Inoue, S., M. Sugai, Y. Murooka, S.-Y. Paik, Y.-M. Hong, H. Ohgai, and H. Suginaka. 1991. Molecular cloning and sequencing of the epidermal cell differentiation inhibitor gene from Staphylococcus aureus. Biochem.Biophys.Res.Commun. 174:459-464.
26.Jaffe, J., S. Natanson-Yaron, M.G. Caparon, and E. Hanski. 1996. Protein F2, a novel fibronectin-binding protein from Streptococcus pyogenes, possesses two binding domains. Molec.Microbiol. 21:373-384.
27.Johnsson, H., H. Lindmark, and B. Guss. 1995. A protein G-related cell surface protein in Streptococcus zooepidemicus. Infect.Immun. 63:2968-2975.
28.Jonsson, K., C. Signas, H.-P. Muller, and M. Lindberg. 1991. Two different genes encode fibronectin binding proteins in Staphylococcus aureus. Eur.J.Biochem. 202:1041-1048.
29.Kao, S.-M., S.B. Olmsted, A.S. Viksnins, J.C. Gallo, and G.M. Dunny. 1991. Molecular and genetic analysis of a region of plasmid pCF10 containing positive control genes and structural genes encoding surface proteins involved in pheromone-inducible conjugation in Enterococcus faecalis. J.Bacteriol. 173:7650-7664.
30.Kastern, W., U. Sjobring, and L. Bjorck. 1992. Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain. J.Biol.Chem. 267 (18):12820-12825.
31.Katsukawa, C. 1994. Cloning and nucleotide sequence of type 3 M protein gene (emm3) consisting of an N-terminal variable portion and C-terminal conserved C repeat regions: relation to other genes of Streptococcus pyogenes. Journal of Japanese Association for Infectious Diseases 68:698-705.
32.Kelly, C., P. Evans, L. Bergmeier, S.F. Lee, F.A. Progulske, A.C. Harris, A. Aitken, A.S. Bleiweis, and T. Lerner. 1990. Sequence analysis of a cloned streptococcal surface antigen I/II. FEBS Lett. 258:127-132.
33.Kline, J. B., Xu, S., Bisno, A. L., and Collins, C. M. Identification of a fibronectin-binding protein (GfbA) in pathogenic group G streptococci. Infect.Immun. 64(6), 2122-2129. 1996.
34.Kok, J., K.J. Leenhouts, A.J. Haandrikman, A.M. Ledeboer, and G. Venema. 1988. Nucleotide sequence of the cell wall proteinase gene of Streptococcus cremoris Wg2. Appl.Environ.Microbiol. 54:231-238.
35.Kreikemeyer, B., S.R. Talay, and G.S. Chhatwal. 1995. Characterization of a novel fibronectin-binding surface protein in group A streptococci. Molec.Microbiol. 17:137-145.
36.Lindgren, P.-E., M.J. McGavin, C. Signas, B. Guss, S. Gurusiddappa, M. Hook, and M. Lindberg. 1993. Two different genes coding for fibronectin-binding proteins from Streptococcus dysgalactiae. The complete nucleotide sequences and characterization of the binding domain. Eur.J.Biochem. 214:819-827.
37.Manjula, B.N., K.M. Khandke, T. Fairwell, W.A. Relf, and K.S. Sripakash. 1991. Heptad motifs within the distal subdomain of the coiled-coil rod region of M protein from rheumatic fever and nephritis associated serotypes of group A streptococci are distinct from each other: nucleotide sequence of the M57 gene and relation of the deduced amino acid sequence of other M proteins. J.Protein Chem. 10:369-383.
38.McDevitt, D., P. Francois, P. Vaudaux, and T.J. Foster. 1994. Molecular charcterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus. Molecular Microbiology 11(12):237-248.
39.Michel, J.L., L.C. Madoff, K. Olson, D.E. Kling, D.L. Kasper, and F.M. Ausubel. 1992. Large, identical, tandem repeating units in the C protein alpha antigen gene, bca, of group B streptococci. Proc.Natl.Acad.Sci.U.S.A. 89:10060-10064.
40.Miller, L., L. Gray, E.H. Beachey, and M.A. Kehoe. 1988. Antigenic variation among group A streptococcal M proteins: Nucleotide sequence of the serotype 5 M protein gene and its relationship with genes encoding types 6 and 24 M proteins. J.Biol.Chem. 263:5668-5673.
41.Mouw, A.R., E.H. Beachey, and V. Burdett. 1988. Molecular evolution of streptococcal M protein: Cloning and nucleotide sequence of type 24 M protein gene and relation to other genes of Streptococcus pyogenes. J.Bacteriol. 170:676-684.
42.Nilsson, A., L. Frykberg, J.-I. Flock, L. Pei, M. Lindberg, and B. Guss. 1998. A fibrinogen-binding protein of Staphylococcus epidermis. Infection and Immunity 66:2666-2673.
43.Nilsson, M., L. Frykberg, J.I. Flock, L. Pei, M. Lindberg, and B. Guss. 1998. A fibrinogen-binding protein of Staphylococcus epidermidis. Infect.Immun. 66:2666-2673.
44.O'Toole, P., L. Stenberg, M. Rissler, and G. Lindahl. 1992. Two major classes in the M protein family in group A streptococci. Proc.Natl.Acad.Sci.U.S.A. 89:8661-8665.
45.Okahashi, N., C. Sasakawa, S. Yoshikawa, S. Hamada, and T. Koga. 1989. Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans implicated in dental caries. Molec.Microbiol. 3:673-678.
46.Olsson, A., M. Eliasson, B. Guss, B. Nilsson, U. Hellman, M. Lindberg, and M. Uhlen. 1987. Structure and evolution of the repetitive gene encoding streptococcal protein G. Eur.J.Biochem. 168:319-324.
47.Pancholi, V. and V.A. Fischetti. 1993. Glyceraldehyde-3-phosphate dehydrogenase on the surface of group A streptococci is also an ADP-ribosylating enzyme. Proc.Natl.Acad.Sci.U.S.A. 90 :8154-8158.
48.Pancholi, V. and V.A. Fischetti. 1998. a-enolase, a novel strong plasmin(ogen) binding protein of the surface of pathogenic streptococci. J.Biol.Chem. 273:14503-14515.
49.Patti, J.M., H. Jonsson, B. Guss, L.M. Switalski, K. Wiberg, M. Lindberg, and M. Hook. 1992. Molecular characterizations and expression of a gene encoding a Staphylococcus aureus collagen adhesin. J.Biol.Chem. 267:4766-4772.
50.Rakonjac, J.V., J.C. Robbins, and V.A. Fischetti. 1995. DNA sequence of the serum opacity factor of group A streptococci: Identification of a fibronectin-binding repeat domain. Infect.Immun. 63:622-631.
51.Rathsam, C., P.M. Giffard, and N.A. Jacques. 1993. The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system. J.Bacteriol. 175:4520-4527.
52.Robbins, J.C., J.G. Spanier, S.J. Jones, W.J. Simpson, and P.P. Cleary. 1987. Streptococcus pyogenes type 12 M protein regulation by upstream sequences. J.Bacteriol. 169:5633-5640.
53. Rocha, C and Fischetti, V. A. 1999. Isolation and characterization of a fibronectin binding protein on the surface of group A streptococci. Infect. Immun. (Press)
54. Schneewind, O., K.F. Jones, and V.A. Fischetti. 1990. Sequence and structural characterization of the trypsin-resistant T6 surface protein of group A streptococci. J.Bacteriol. 172:3310-3317.
55.Sela, S., A. Aviv, A. Tovi, I. Burstein, M.G. Caparon, and E. Hanski. 1993. Protein F: an adhesin of Streptococcus pyogenes binds fibronectin via two distinct domains. Molec.Microbiol. 10(5):1049-1055.
56.Signas, C., G. Raucci, K. Jonsson, P. Lindgren, G.M. Anantharamaiah, M. Hook, and M. Lindberg. 1989. Nucleotide sequence of the gene for fibronectin-binding protein from Staphylococcus aureus: Use of this peptide sequence in the synthesis of biologically active peptides. Proc.Natl.Acad.Sci.U.S.A. 86:699-703.
57.Sjobring, U. 1992. Isolation and molecular characterization of a novel albumin-binding protein from group G streptococci. Infect.Immun. 60:3601-3608.
58.Smith, H.E., U. Vecht, A.L.J. Gielkens, and M.A. Smits. 1992. Cloning and nucleotide sequence of the gene encoding the 136-kilodalton surface protein (muramidase-released protein) of Streptococcus suis type 2. Infect.Immun. 60:2361-2367.
59.Suvorov, A.N., A.E. Flores, and P. Ferrieri. 1997. Cloning of the glutamine synthetase gene from group B streptococci. Infect.Immun. 65:191-196.
60.Talay, S.R., M.P. Grammel, and G.S. Chhatwal. 1996. Structure of a group C streptoccal protein that binds to fibrinogen, albumin and immunoglobulin G via overlapping modules. Biochem.J. 315:577-582.
61.Talay, S.R., P. Valentin-Weigand, P.G. Jerlstrom, K.N. Timmis, and G.S. Chhatwal. 1992. Fibronectin-binding protein of Streptococcus pyogenes: sequence of the binding domain involved in adherence of streptococci to epithelial cells. Infect.Immun. 60:3837-3844.
62.Timoney, J.F., S.C. Artiushin, and J.S. Boschwitz. 1997. Comparison of the sequences and functions of Streptococcus equi M-like proteins SeM and SzPSe. Infect.Immun. 65:3600-3605.
63.Tokuda, M., N. Okahashi, I. Takahashi, M. Nakai, S. Nagaoka, M. Kawagoe, and T. Koga. 1991. Complete nucleotide sequence of the gene for a surface protein antigen of Streptococcus sobrinus. Infect.Immun. 59:3309-3312.
64.Wanda, S.-Y. and R. Curtiss III. 1994. Purification and characterization of Streptococcus sobrinus dextranase produced in recombinant Escherichia coli and sequence analysis of the dextranase gene. J.Bacteriol. 176(13):3839-3850.
65.Weidlich, G., R. Wirth, and D. Galli. 1992. Sex pheromone plasmid pADI-encoded surface exclusion protein of Enterococcus faecalis . Mol.Gen.Genet. 233:161-168.
66.Yeung, M.K. and J.O. Cisar. 1988. Cloning and nucleotide sequence of a gene for Actinomyces naeslundii 45 type 2 fimbriae. J.Bacteriol. 170:3803-3809.
67.Yeung, M.K. and J.O. Cisar. 1990. Sequence homology between the subunits of two immunologically and functionally distinct types of fimbriae of Actinomyces spp. J.Bacteriol. 172:2462-2468.
68.Yeung, M.K., J.A. Donkersloot, J.O. Cisar, and P.A. Ragsdale. 1998. Identification of a gene involved in assembly of Actinomyces naeslundii T14V type 2 fimbriae. Infection and Immunity 66:1482-1491.
69.Yother, J. and D.E. Briles. 1992. Structural properties and evolutionary relationships of PspA, a surface protein of Streptococcus pneumoniae, as revealed by sequence analysis. J.Bacteriol. 174:601-609.
70. Scott, J.R. and D. Zähner. 2006. Pili with strong attachments: Gram-positive bacteria do it differently. Molec Microbiol 62:320–330.

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