Phage Lysins

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Phage Lytic Enzymes Currently Developed in the Fischetti Lab

Lysin Bacteria digested by lysin Publications
PlyC S. pyogenes
S. uberis
S. equi
[1-3]
PlyGBS Group B streptococci (all serotypes)
S. pyogenes
Group D streptococci
Group L streptococci
S. salivarius
[4;5]
Cpl-1, PAL S. pneumoniae (all capsular types) [6-14]
PlyV12 E. faecalis (VRE)
E. faecium
S. pyogenes
Groups B, C, E, F, L, N streptococci
S. uberis
S. gordinii
S. intermedius
S. parasanguis
S. aureus
[15]
ClyS S. aureus (MRSA, VISA) & (all other staphylococci)
PlyB B. anthracis B. cereus B. thuringiensis B. megaterium
PlyG B. anthracis [16]
PlyPH B. anthracis (broad pH range of activity) [17]

Publication List

  1. Nelson D, Loomis L, Fischetti VA: Prevention and elimination of upper respiratory colonization of mice by group A streptococci by using a bacteriophage lytic enzyme. Proc.Natl.Acad.Sci.U.S.A 2001, 98:4107-4112.
  2. Nelson D, Schuch R, Zhu S, Tscherne DM, Fischetti VA: Genomic sequence of C1, the first streptococcal phage. Journal of Bacteriology 2003, 185 (11):3325-3332.
  3. Nelson D, Chahalis P, Zhu S, Fischetti V: PlyC: The first multimeric bacteriophage lysin. Proc.Natl.Acad.Sci. 2006.
  4. Cheng Q, Nelson D, Zhu S, Fischetti VA: Removal of group B streptococci colonizing the vagina and oropharynx of mice with a bacteriophage lytic enzyme. Antimicrobial Agents and Chemotherapy 2005, 49:111-117.
  5. Cheng Q, Fischetti VA: Mutagenesis of a bacteriophage lytic enzyme PlyGBS significantly increases its lytic activity. J Bacteriol 2006.
  6. Systemic use of the endolysin Cpl-1 rescues mice with fatal pneumococcal pneumonia`. Critical Care Medicine, In press 2008.
  7. McCullers JA, Karlstrom A, Iverson AR, Loeffler JM, Fischetti VA: Novel strategy to prevent otitis media caused by colonizing Streptococcus pneumoniae. PLoS.Pathog. 2007, 3:e28.
  8. Loeffler JM, Nelson D, Fischetti VA: Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase. Science 2001, 294:2170-2172.
  9. Loeffler JM, Djurkovic S, Fischetti VA: Phage lytic enzyme Cpl-1 as a novel antimicrobial for pneumococcal bacteremia. Infect.Immun. 2003, 71:6199-6204.
  10. Loeffler JM, Fischetti VA: Synergistic lethal effect of a combination of phage lytic enzymes with different activities on penicillin-sensitive and -resistant Streptococcus pneumoniae strains. Antimicrob Agents Chemother. 2003, 47:375-377.
  11. Grandgirard D, Loeffler JM, Fischetti VA, Leib SL: Phage lytic enzyme cpl-1 for antibacterial therapy in experimental pneumococcal meningitis. J.Infect.Dis. 2008, 197:1519-1522.
  12. Entenza JM, Loeffler JM, Grandgirard D, Fischetti VA, et al.: Therapeutic effects of bacteriophage Cpl-1 lysin against Streptococcus pneumoniae Endocarditis in rats. Antimicrobial Agents and Chemotherapy 2005, 49:4789-4792.
  13. Entenza JM, Loeffler JM, Grandgirard D, Fischetti VA, Moreillon P: Therapeutic effects of bacteriophage Cpl-1 lysin against Streptococcus pneumoniae endocarditis in rats. Antimicrob.Agents Chemother. 2005, 49:4789-4792.
  14. Djurkovic S, Loeffler JM, Fischetti VA: Synergistic killing of Streptococcus pneumoniae with the bacteriophage lytic enzyme Cpl-1 and penicillin or gentamicin depends on the level of penicillin resistance. Antimicrob Agents Chemother 2005, 49:1225-1228.
  15. Yoong, P., R. Schuch, D.C. Nelson and V.A. Fischetti. Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faecium. Journal of Bacteriology 2004, 186:4808-4812.
  16. Schuch R, Nelson D, Fischetti VA. A bacteriolytic agent that detects and kills Bacillus anthracis. Science 418, 884-889. 2002.
  17. Yoong, P., R. Schuch, D. Nelson and V. A. Fischetti. PlyPH, a bacteriolytic enzyme with a broad pH range of activity and lytic action against Bacillus anthracis. J.Bacteriol 2006, 188:2711-2714.