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Olis RSM1000F with U.S.A. Stopped-Flow Spectrofluorimeter

Emission scan rates to 1,000 scans/second are achievable on this system that uses a 450 watt xenon arc lamp as the exciting source, a single grating monochromator as the exciting light wavelength selector, and the RSM with a mid-plane PMT. Issues with the bright 450 watt xenon arc lamp - heat and stray light - were addressed by new components.

Key Features:

Wavelength Range:	180-1200 nm
Stray Light:	Sister Monochromator: Featuring a 40x45 mm concave holographic grating driven by a small servo motor; the unit has conventional entrance and exit slits and a place for a second-order filter; provides high light throughput, affords the equivalent wavelength range of scores of filters, and can be adjusted using a simple keystroke command to the software to select excitation wavelengths. Middle-Plane PMT: Optimizes photosensitivity; attached to a trap-door lid, this high-gain, side-window PMT intercepts the emission beam at the middle plane of the RSM, maximizing detection of weak luminescence.
Light Source:	450 watt xenon arc lamp
Experimental Modes:	- Rapid-scanning: 1,000 emission spectral scans per second - Conventional scanning - Fixed wavelength: microsecond read rates for monitoring very fast kinetic
Stopped-flow features:	- Deadtime: 1-2 ms for absorbance and fluorescence (3-5 ms for circular dichroism) - Average Reagent Volume: 70 -120 �l/syringe/shot - Low Volume Design: 40-70 �l/syringe/shot - Pathlength: classic ambient-temperature: 20 mm for absorbance, 10 mm for fluorescence; low-temperature fluorescence: 4 mm; low-temperature absorbance: 10 mm - Mixer: Berger-Ball - Drive syringes: Unimetrics® glass syringes; low-temperature syringes also available. - Valves: ceramic (i.e., fired sapphire) - Tubing from valves to observation cell: user's choice of Tefzel® or stainless steel; from observation cell to waste syringe: Teflon®. - Flow tubes: quartz for absorbance and fluorescence models, stainless steel for low-temperature model. - Syringe extenders: PEEK® (polyetheretherketone), a polymer inert to almost all organic solvents. - Large, convenient knobs and dials - Fitted with accessible ports with easy on/off fitting of water and gas lines to bath chamber for thermostatting and degassing of bath fluid and exposed syringe plungers. - Floodable water bath chamber is made of Delrin®, a dense (1.56g/cm3) polymer. - Using syringes of different sizes provides reagent ratios to 1:11. - Pneumatic drive system; one plunger for both syringes; independent movement of syringes and valves for convenience in filling and flushing. - Safety interlock system for 100% assurance against misfiring, active with and without Olis computer control. - Anodized cast-aluminum channel (0.5 cm thick) and optical railing (1 cm thick) for high structural integrity. - Fail-safe alignment of stopped-flow observation ports within sample compartment of host spectrometer
Chiller:	Closed-cycle cooling box: Cools both the lamp housing and the output beam.