Evening Workshops for Professional Development

PCR 101

Instructor: Melissa Lee, Harlem DNA Lab

Dates: October 28-29
Time: 5pm - 7pm
Location: The Rockefeller University Science Outreach Lab  
Limit: 15 Participants 
Cost: Free 

Polymerase chain reaction (PCR) is a common and often indispensable technique used by researchers to produce millions of copies of a specific DNA sequence in a short period of time. This automated process can be used for a variety of applications, including DNA fingerprinting in forensics and paternity testing, diagnosis of genetic diseases, and analyses of genes.

Participants of this course will extract their own DNA and analyze their DNA using PCR and gel electrophoresis. PCR will be used to amplify a region of DNA on chromosome 16 that is variable between individuals. Chromosome 16 may contain an insertion of extra DNA called an Alu insertion. The presence or absence of this insertion plays no role in the health or well-being of a human because it is located in "junk" DNA. Individuals can inherit one insertion (from one parent), two insertions (from both parents) or no insertions at all. These differences can be observed using gel electrophoresis.

In addition to learning about the process of PCR, participants will be given an opportunity to discuss strategies for implementing PCR in the classroom.

Please sign up for this workshop using the form below.