We have access to high-end Bruker Avance DMX NMR spectrometers at field strengths of 400, 500, and 600 MHz in the NMR Spectroscopy Facility at Rockefeller Campus. A recent installation of CryoProbe on the 600 spectrometer provides 3-4 fold gains in sensitivity, allowing us to study large, low sensitivity protein complexes. We also have access to the cutting-edge NMR spectrometers at the New York Structural Biology Center (NYSBC), including high-field 800 and 900 MHz spectrometers equipped with CryoProbes. We study the structures, dynamics and functions of proteins and proteins complexes using various advanced NMR techniques including heternuclear triple-resonance experiments with sensitivity enhancement using pulse-field gradients, three-dimensional heteronuclear NOE experiments, TROSY-based triple-resonance experiments with 2H decoupling, and residual dipolar coupling (RDC) measurements.