We study nuclear pore complexes (NPCs), which provide the only known sites for nucleocytoplasmic exchange. Using a variety of techniques and yeast as a model system, we are determining the composition and fine structure of the NPC and relating this to its sites of interactions and reactions with soluble nuclear transport factors. We are also examining associations between NPCs and other components of the nuclear periphery. We hope to gain a better understanding of the part the NPC plays in nuclear regulation and maintenance.
