Video Microscopy of Neuronal Migration - Movies from the Hatten Lab
Solecki, 2003
Centrosomal and nuclear motion in a migrating neuron. Purified cerebellar granule neurons were labeled with Ven-mPar6 alpha and cultured with cerebellar glia. Movement of the centrosome precedes nuclear movement as the neuron migrates along it glial guide. Total elapsed time was six minutes.
Hatten, Bhatt, Tomoda, Didkovsky, 2000
Migrating Granule Cell in vivo
Hatten, Bhatt, Tomoda, Didkovsky, 2000
Migrating Granule Cell in vivo
Hatten, Bhatt, Tomoda, Didkovsky, 2000
Parallel Fiber Extension in situ
Hatten, Wingate, Didkovsky, 2000
Migrating granule cells
Millonig, Gallagher, Didkovsky, 2000
Migrating granule cells
(MPEG movie, 1.31MB)
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(GIF89a movie, 736KB)
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Hatten and Edmondson, 1987. (J. Neurosci.)
Migration of cerebellar granule cells along astroglial fibers in vitro. Immature granule neurons, purified
from early postnatal cerebellum, extend a specialized, migratory process along the underlying glial fiber. In this sequence,
a neuron moves along a glial fiber at approximately 50 microns/h. As
the neuron moves, lamellipodia and filopodia extend and retract along the length of the migratory process, enwrapping
the glial guide. The neuron forms an interstitial junction along the length of the cell soma, which is released
as the cell begins to move.
(MPEG movie, 528KB)
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(GIF89a movie, 554KB)
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Gasser and Hatten, 1989. (Proc. Natl. Acad. Sci USA)
Migration of neurons from one brain region along astroglial fibers from another. Immature granule neurons, purified from
the early postnatal cerebellar cortex, migrate along the processes of glial cells isolated from hippocampus. The dynamics of
movement of neurons from one region closely parallel those of neurons from other regions, suggesting that the mode of
migration along glial fibers is stereotyped. The movement of the neuron is saltatory. The leading process extends along
the glial fiber, after which the cell soma appears to contract and then extend just prior to release of the adhesion junction
underneath the cell soma. This sequence is repeated, as the neuron progresses along the glial fiber.
(MPEG movie, 719KB)
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(GIF89a movie, 736KB)
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Fishell and Hatten, 1991. (Development)
Astrotactin Provides a Neuronal Ligand for Movement along the Glial Fiber. To provide an assay for cell surface receptor systems
that function in neuronal migration, low power views are used to track the movement of a large population of cerebelar granule cells.
In the first sequence, neurons are seen migrating along glial fibers. The application of antibodies against the neuronal protein
astrotactin (flash across the top of the screen) results in rapid arrest of neuronal movement. As migration ceases, the long glial processes
"sway" across the field.
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