Rockefeller University

The Rockefeller University
1230 York Avenue
New York, NY 10021

Welcome to The Gadsby Lab
Laboratory of Cardiac/Membrane Physiology

D.W. Bronk Building, Room 307
Tel: (212)327-8617 Fax: (212)327-7589

 

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Molecular mechanisms of ion transport across cell membranes

The main focus of the laboratory is on electrophysiological investigation of molecular mechanisms of ion transport across cell membranes, and their regulation by cellular signaling pathways.

We mostly study two molecular machines: the sodium/potassium pump (Na+, K+-ATPase), which transports hundreds of Na and K ions across the cell membrane every second, and the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) chloride channel, whose open conformation allows millions of chloride ions to cross the membrane each second. Both are ATPases and harness the free energy of ATP binding and hydrolysis to generate the conformational changes that control ion transport. We use mutagenesis, and biochemical and high-resolution electrical methods to examine those conformation changes, sometimes in one molecule at time.

11th. International ATPase Conference link Homology model of a CFTR NBD1/NBD2 Structure of SERCA Ca2+-ATPase homolog of Na+,
	K+-ATPase subunit
11th. International ATPase Conference
September 6-11, 2005 MBL,Woods Hole

( cartoon by C.Toyoshima)
Homology model of a CFTR NBD1/NBD2 dimer based on the MJ0796 structure (Smith et al. , Mol Cell. 10(1):139-49, 2002) by Martin Mense * Structure of SERCA Ca2+-ATPase homolog of Na+, K+-ATPase subunit (from Toyoshima et al.,Nature.405:647-55,2000) *
* Click on image for high resolution movie

Send question or comments to David Gadsby (gadsby@mail.rockefeller.edu)


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Last Update February, 2005