Cells deleted for all G1 cyclins CLN1, CLN2 and CLN3 but provided with a methionine-regulatable MET3-CLN2 gene were periodically pulsed with methionine-free medium in the microfluidics flow-cell device of Charvin et al.( PLoS ONE. 2008 Jan 23;3(1):e1468). Green: Whi5-GFP; G1/S transcriptional repressor. Yellow: CDC10-YFP: marker of bud neck, appears at budding and disappears at cytokinesis. Bottom: bars indicate periods of induction with -Met.