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DNA/RNA Sample Preparation and Quality Control

DNA/RNA Sample Preparation and Quality Control

It's very important that users provide us DNA or RNA samples at required quality and quantity. Please refer to the Services section for specific requirements of sample submission. Here are some general guidelines for sample preparation and quality control:

Total RNA preparation

RNA quantitation

We recommend the NanoDrop spectrophotomer for quick quantitation. NanoDrop at our center is available free of charge.

RNA quality assessment

The quality of total RNA samples is very important in RNA-Seq and microarray experiments. When you provide us total RNA samples, we always perform RNA integrity check on Agilent 2100 Bioanalyzer.  RNA integrity number (RIN) at 8.0 or higher is required by most protocols. 

Genomic DNA Preparation for SNP genotyping and sequencing

DNA quantitation

Genomic DNA Quality Assessment:

DNA/RNA Sample Preparation and Quality ControlIt's very important that users provide us DNA or RNA samples at required quality and quantity. Please refer to the Services section for specific requir