Buffer Suggestions for the Cell Sorting

Purpose and DetailsComponentsBasic Cell Sorting BufferVariations of the Basic Cell Sorting BufferCell Sorting Buffer for Clean Lymphoid Cells
For Sticky CellsFor Adherent CellsFor Samples with a Lot of Dead Cells
Increase buffer capacity and stability
1x Phosphate Buffered Saline
(DPBS, Ca/Mg++ free),
pH 7.0-7.4
checkmark checkmark checkmark checkmark  
HEPES pH 7.0 (10-25 mM) checkmark checkmark checkmark checkmark  
HBSS,
with Calcium/Magnesium,
no Phenol Red
        checkmark
Chelating agent to prevent cell aggregation
EDTA (~ 5mM or 0.5%)   checkmark checkmark    
Protein to maintain cell viability
BSA (Bovine Serum Albumin,
0.1-1%) or
cation-free-FBS or FCS
(dialyzed against Ca/Mg++ free DPBS, 1-5%)
checkmark checkmark checkmark checkmark  
FBS-HI (Fetal Bovine Serum Heat-Inactivated, 1%)         checkmark
Digestion free DNA
DNAse-I (RNAse free) (10U/ml or 25-50ug/ml);
add MgCl2 (1-5mM)
      checkmark  
Sterility
Sterilize by 0.2um filtration,
store at 4°C
checkmark checkmark checkmark checkmark checkmark
Additional Notes       Usecation-freeFBS in order to stop the trypsin reaction Keep DNAse in all the buffers through the sample preparation