Imagestream-X Default Filter Configuration
| Laser | Detector Name | Equivalent Bandpass Filter | Sample Parameters / Fluorochromes |
|---|---|---|---|
| Blue - Yellow |
Channel 1 | 450/60 | Brightfield |
| Channel 2 | 520/80 |
FITC; Alexa 488; GFP; YFP; CFSE; SYTOX Green; LIVE/DEAD Fixable Green; DyeCycle Green; Azami Green (FUCCI) |
|
| Channel 3 | 578/35 | PE; DSRed FP | |
| Channel 4 | 628/65 | PE-Alexa610; PE-TxRd; PI; 7AAD; mCherry; TagRFP; Kusabira Orange (FUCCI) |
|
| Channel 5 | 700/80 | PerCP; PE-Cy5.5; PerCP-Cy5.5; PE-Alexa700; DRAQ5 | |
| Channel 6 | 770/60 | Darkfield (SSC); PE-Cy7; PE-Alexa750; PE-eFluor780 | |
| Violet - Red |
Channel 7 | 463/85 | Pacific Blue; Alexa405; Marina Blue; Cascade Blue; DyeCycle Violet; SYTOX Blue; CellTrace calcein Violet AM; LIVE/DEAD Fixable Violet, DAPI |
| Channel 8 | 538/65 | LIVE/DEAD Aqua; Qdot 525; Qdot 545; Pacific Orange | |
| Channel 9 | 583/25 | Brightfield sync; Qdot 565; Qdot 585 | |
| Channel 10 | 628/65 | Q-dot 605; Qdot 625 | |
| Channel 11 | 700/80 | Alexa647; APC; Cy5; Alexa680; Alexa700; SYTOX Red | |
| Channel 12 | 770/60 | APC-Cy7; APC-Alexa750; APC-eFluor780 |