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Flow Cytometry Resource Center (FCRC)
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Instruments
For analysis purposes, the FCRC is equipped with benchtop alignment-free multipurpose analyzers from BD Immunocytometry Systems (FACSort, FACS Calibur and BD LSR). The laser configurations on these instruments allow for analysis of cell samples stained with 488 nm, 633 nm and 325 nm (UV) excited dyes. A number of applications, including the multicolor analysis of cell phenotype, gene expression and cell cycle may be performed. After orientation training, the analyzers are available for use directly by the investigators.

For cell sorting, the FCRC is equipped with a FACS Vantage SE Sorter (BD Immunocytometry Systems). This instrument provides high-speed, high purity (up to 99.0%) multilaser cell sorting and is capable of sterile sorts of single cells directly into culture plates. The sorter is operated by FCRC staff only.


Instrument Year
of
Manu-
facture
Fee
$/
hour
Hours
of
Operation
Purpose of Use Lasers (excitation beam) Number
of
detectors

(by
laser)
Cell
Sort
Data

Acqui-
sition
Data

Anal-
ysis
Data

Sto-
rage
Pre-set
configuration (nm)
Option
to
change
wave-
length
(nm)
I
Laser
II
Laser
III
Laser
IV
Laser
FACS
Vantage
SE with
DiVa
option
1991/
2003
64/86 Regular
Business
Hours
yes yes yes yes 488 647 408 no III
Laser-
350,408,
413,457
8
(4/2/2)
BD
FACSAria
2005 64/86 Extended
Business
Hours
yes yes yes yes 488 633 405 no no 12
(7/3/2)
BD LSR II-1 2004 32 24/7 no yes yes no 488 405 355 635 no 13
(5/3/2/3)
BD LSR II-2 2007 32 24/7 no yes yes no 488 405 561 640 no 16
(5/3/5/3)
FACS
Calibur I
2002 22 24/7 no yes yes no 488 no no no no 3 (3)
FACS
Calibur II
2003 22 24/7 no yes yes no 488 635 no no no 4 (3/1)
FACSort 1993 22 24/7 no yes yes no 488 635 no no no 4 (3/1)
FCRC
Analysis I
2002 no 24/7 no no yes no N/A N/A N/A N/A N/A N/A
FCRC
Analysis II
2000 no 24/7 no no yes no N/A N/A N/A N/A N/A N/A
FCRC
Analysis
PC
2005 no 24/7 no no yes no N/A N/A N/A N/A N/A N/A