Guidelines

To help us maintain the Flow Cytometry Resource Center at its optimum, we ask that you read and follow these guidelines and be considerate of other users. If you have questions or requests, need advice on procedures or have any suggestions or concerns, please do not hesitate to contact FCRC Director at any time.

  1. RESOURCE AVAILABILITY.
    1. Cell Sorters - BD FACSAriaII-1, BD FACSAriaII-2, and BD FACS AriaII-3.
      1. The instruments and operators are available for sorts Monday through Friday according to the Cell Sorting Schedule.
      2. The sorts are scheduled on a first-booked-first-served basis.
      3. Cell Sort Scheduling is done at the discretion of the FCRC, to maximize availability for the entire RU community. FCRC Staff schedules sorts from the beginnings or the ends of the day to avoid breaking large time blocks.
      4. If time is unscheduled one day prior to sort, FCRC Staff can adjust the schedule to fit better particular user needs.
      5. There is no time limit for cell sorting (length). Reservation can be made up to 2 months in advance.
      6. To schedule a sort, please:
      7. Prior to the first sort of a new protocol, please arrange an appointment with FCRC Director to discuss the details of the sort.
      8. All the sorts are performed by FCRC operators only.
      9. Condition of NYSTEM funding for the FACSAria II-2:
        • Support through the NYSTEM grant shall be acknowledged in all publications, presentations and products of research resulting from use of or data acquired through the BD FACSAriaII-2, in a form consistent with the publication's guidelines.
        • The acknowledgement should reference "Support by the Empire State Stem Cell fund through NYSDOH Contract #C023046" and include following statement "Opinions expressed here are solely those of the author and do not necessarily reflect those of the Empire State Stem Cell Fund, the NYSDOH, or the State of NY."
        • Please be sure to send to fcrc@rockefeller.edu documentation for publications funded wholly or in part by the contract that include an acknowledgment of this funding support. This information must be submitted to NYSTEM within 60 days of publication.
    2. Imaging Flow Cytometer – ImageStream-X
      1. Sample Acquisition is scheduled on a first-booked-first-served basis.
      2. Sample Acquisition Scheduling is done at the discretion of the FCRC, to maximize availability for the entire RU community. FCRC Staff schedules acquisition from the beginnings or the ends of the day to avoid breaking large time blocks.
      3. If time is unscheduled one day prior to Sample Acquisition, FCRC Staff can adjust the schedule to fit better particular user needs.
      4. There is no time limit for Sample Acquisition (length). Reservation can be made up to 2 months in advance.
      5. To schedule a Sample Acquisitions, please:
      6. Prior to the first Sample Acquisition of a new protocol, please arrange an appointment with FCRC Staff to discuss the details of the sort.
      7. All the Sample Acquisitions are performed by FCRC operators only.
    3. BASIC (BD Accuri® C6) and ADVANCED (BD LSRIIs and BD LSR-Fortessa) Cell Analyzers.
      1. The Analyzers are available 24/7 except during maintenance periods (see Section B. Weekly Maintenance below).
      2. Data acquisition on the Flow Cytometry Cell Analyzers is done by researchers and considered as self-service.
      3. FCRC-PPMS System allows users to book time on the Cell Analyzers:
        • Time could be booked up to two weeks in advance
        • The experiments are scheduled on a first-booked-first-served basis
        • For the time booked more than 24 hours in advance, you may schedule in 30-minute increments and generally should not book more than 2 hours at a time during the high demand period (weekdays: 9:00 am - 6:00 pm)
        • For the short term booking (less than 24 hours in advance), there is no restriction on how much time to be booked
        • If your procedure requires more than 2 hours, please sign-up for non-peak hours (when there is no maximum time restriction) or contact FCRC staff to book time for you
      4. During the acquisition, the data files could be saved in the temporary folder on the desktop of the computer attached to the instrument. After the completion of the experiment, all the files should be moved out (Refer to Data Storage and Retention Policy).
      5. FCRC is not responsible for data files left on FCRC computers.
      6. All the experiments are performed by researchers themselves unless FCRC personnel assistance is requested.
      7. To get an appropriate assistance from FCRC staff please be sure to schedule it in advance by contacting FCRC staff.
      8. For charges of FCRC staff assistance, please refer to Charges and Fees.
    4. Analysis Computers - FCRC MAC Analysis-1, FCRC MAC Analysis-2, FCRC Analysis PC1, FCRC Analysis PC2 and FCRC Analysis PC3 (other than these attached to the instruments above).
      1. The computers are available 24/7.
      2. Could be booked on FCRC-PPMS System. Access to the computers is on first-come-first-served basis (without signup).
      3. These computers are dedicated to flow cytometry data analysis only. They are on the RU network, run Flow Cytometry (CellQuest Pro, FlowJo, BD FACSDiVa, FCS Express, ModFit) and Office programs to analyze and publish data.
      4. Absolutely no data can be stored on the FCRC MAC Analysis-1, FCRC MAC Analysis-2, FCRC Analysis PC1 and FCRC Analysis PC2 machines.
      5. FCRC Analysis PC3 is dedicated for ImageStream-X Data analysis, and data can be temporarily saved for 3 months in this computer. Users are responsible to make archival copies of their data.
      6. After the completion of the experiment, all the files should be moved out (Refer to Data Storage and Retention Policy).
      7. All the data analysis is performed by researchers themselves unless FCRC personnel assistance is requested.
      8. To get an appropriate assistance from FCRC staff please be sure to schedule it in advance by contacting FCRC staff.
      9. For charges of FCRC staff assistance, please refer to Charges and Fees.
  2. WEEKLY MAINTENANCE.
    1. Regular weekly maintenance on all FCRC Analyzers is scheduled on Mondays (see time blocks on FCRC-PPMS System).
    2. Regular weekly maintenance includes the following procedures on all FCRC Analyzers:
      1. Instrument cleaning according to BD-recommended procedures for Weekly or Monthly Maintenance
      2. Quality Control according to BD-recommended procedures
      3. Fresh preparation of all the solutions for spray and drop bottles
      4. Removal and discard of all unlabeled sample tubes and racks from the desks and benches
      5. Purge of all illegitimate files from the computers
  3. CHARGES AND CANCELLATION POLICY.
    1. To recover essential operating costs, the FCRC charges hourly fees for the sorts, ImageStream-X and use of the analyzers according to Charges and Fees.
    2. Usage of computers (other than those attached to the instruments) is free of charge.
    3. To avoid cancellation fees, scheduled operator-assisted services (BD FACSAria-1, BD FACSAria-2, BD FACSAria-3 and ImageStream-X) must be cancelled no later than 48 hours prior to the scheduled sort. If the scheduled sort is not cancelled by this time, the FCRC will impose a charge for one-half of total time originally scheduled. For details please refer to Charges and Fees.
    4. Scheduled use of the BASIC Cell Analyzer (BD Accuri® C6) must be cancelled no later than three hours in advance of the scheduled start time. Scheduled use of the ADVANCED Cell Analyzers (BD LSRIIs and BD LSR-Fortessa) must be cancelled no later than 12 hours in advance of the scheduled start time. If the scheduled use of the analyzer is not cancelled by this time, the FCRC will impose a charge for one-half of the original amount of time scheduled.
    5. Charges on Cell Analyzers are calculated based on logged computer time (Windows), using "login/logout" report.
    6. For charges of FCRC staff assistance, please refer to Charges and Fees.
  4. LOGIN SYSTEM.
    1. Please read detailed information about Login System before planning your experiment.
    2. Be sure to set up all necessary logins/passwords in advance according to Login/Password and Training Requirements.
    3. Please use your own login ID only and log off at the earliest convenience to avoid extra charges.
  5. OPERATION PROCEDURES.
    1. All FCRC ADVANCED Cell Analyzers (BD LSRIIs and BD LSR-Fortessa) are run in combination with BD FACSFlow Supply Systems attached to the instruments. To avoid instrument malfunction be sure to read and follow the BD LSRII Maintenance Guidelines.
    2. Each user is responsible to wash instrument after sample acquisition is completed. Detailed notes on washing procedures are posted on the instruments. After instrument is washed, be sure to put it on "standby".
    3. Do not forget to Log off from FCRC computers after the work completed.
    4. During regular business hours (9:00 am - 6:00 pm on weekdays) please leave Instrument "on".
    5. During weekends and off hours (after 6:00 pm on weekdays and all day on weekends and holidays) please switch "off" Instrument.
    6. Never turn "off" FCRC computers to allow remote access for RU IT and scheduled backups to be performed.
    7. Clean up FCRC bench space and discard all the tubes into the red containers for biohazard waste.
    8. Please discard all non-biohazard waste into black containers for regular waste.
  6. TRAINING, CONSULTATION, DISCUSSION AND TROUBLESHOOTING.
    1. The subjects reviewed at the training sessions vary depending on the background, needs and interests of the investigators. See Class and Hands-on Training Requirements. Please do not hesitate to contact FCRC Director to find out the best solution for you.
    2. For the Class and Hands-on Training (BD LSR, HTS, and Accuri® C6), please fill up the specific form. The Class and Training sessions are scheduled on a first-booked-first-served basis.
    3. For Consultation and Help, please go to Flow Cytometry Consultation and Help to fill up the specific form.
    4. For Discussion, feel free to contact the FCRC Director during regular working hours or 24/7 via e-mail.
  7. THE FACILITY.
    1. The FCRC is located in rooms 205, 207 and 211 of the Detlev W. Bronk Building (DWB 205, 207 and 211).
    2. The Sorter room and office (DWB 205 and 207) is unlocked from 9:00 am to 6:00 pm, Monday to Friday. The Analyzer room (DWB 211) is limited to those with authorized University ID cards.
    3. You may have your ID card programmed for access by requesting RU Card Activation for FCRC Door Access. For safety purposes, please do not prop open the door to the FCRC.