|
Please run the complete cleaning procedure every time you finish the acquisition of your samples.
1. Wash the FACS Analyzers (FACSort, FACSCalibur, BD LSR).
- For 5 minutes on Run (HI) with 10% bleach (wash I) from the red drip bottle;
- For 5 minutes on Run (HI) with DD Water (wash II) from the white drip bottle.
- Leave the tube with 1 ml of DD Water (wash II) on STANDBY. (The DD Water should remain in the tube when the instrument is on STANDBY or has been turned off to prevent salt deposits from forming in the sample injection tube.)
2. Refill the sheath reservoir up to no more than 3/4 full (see detailed instructions on the next page). The FACSFlow SHEATH solution is ready to use (Becton Dickinson, Cat. # 342003) and is located by the sink. The additional FACSFlow SHEATH supply is stored under the printer desk. (Ensure that fluid hoses and red wire are not twisted).
3. Empty the waste reservoir (see detailed instructions on the page after the next).
- Empty the waste reservoir into the sink.
- Rinse the waste reservoir with tap water.
- Let the tap water run to rinse the sink for 1-2 minutes.
- Put 200-400 ml of straight bleach into the waste tank. Commercial (96 oz) container with bleach is stored under the sink.
4. Remove all the stuff remaining from your experiment. Wipe the bench top. Quit the CellQuest. LOG OFF the multi-user system.
Filling the SHEATH RESERVOIR:
- If the FACS Analyzer (FACSort or FACSCalibur) is powered on, put it on STANDBY.
- Slide out the fluidics drawer. If the FACS Analyzer is powered on, please do not forget to relieve the pressure by pushing the vent valve toggle switch.
- Slide the black metal bracket away from you, and lift up to remove it.
- Unscrew the cap assembly from the sheath reservoir and set the assembly aside on the clean paper towel, placed on the top of waste reservoir.
- Remove the sheath reservoir.
- Fill the sheath reservoir to _ its capacity with the FACSFlow SHEATH solution located by the sink. To avoid spills and mess, please do it over the sink. (CAUTION: when the sheath reservoir is filled beyond the recommended level, fluid may backflow and damage the instrument.)
- Install the sheath reservoir into the fluidics drawer. (CAUTION: before you install the reservoir back into the fluidics drawer, wipe the outside of the sheath reservoir with clean paper towel to avoid the corrosion of the instrument.)
- Replace and tighten the cap assembly. (CAUTION: try to avoid twists and pinches of the tubing.)
- Replace the black metal bracket.
Always empty the waste reservoir when you fill the sheath reservoir. It will prevent the waste reservoir from overflowing.
Empting the WASTE RESERVOIR:
- Unscrew the cap assembly from the waste reservoir and set the assembly aside on the clean paper towel, placed on the top of sheath reservoir.
- Remove the waste reservoir.
- Empty the waste reservoir into the sink. Let the tap water run for 1-2 minutes. Rinse the waste reservoir with tap water.
- Put 200-400 ml of straight bleach into the waste tank. Commercial (96 oz) container with bleach is stored under the sink.
- Install the waste reservoir back into the fluidics drawer. (CAUTION: before you install the reservoir back into the fluidics drawer, wipe the outside of the waste reservoir with clean paper towel to avoid the corrosion of the instrument.)
- Replace and tighten the cap assembly. (CAUTION: try to avoid twists and pinches of the tubing.)
- If the FACS Analyzer is powered on, please do not forget to pressurize the sheath reservoir by pushing the vent valve toggle switch (follow the arrow sign).
- Slide in the fluidics drawer.
| 
