1. We have two new super-resolution microscope systemsin the BIRC!
    • The OMX 3D-SIM system from Applied Precision performs 3D-Structured Illumination Microscopy on samples up to around 20 microns in thickness, labeled with up to 3 conventional dyes or fluorescent proteins, achieving double the normal resolution in all 3 axes (~120nm in x,y and ~300nm in z). The system was recently upgraded to the "Blaze" version, achieving imaging speeds compatible with certain live cell processes.
    • The Nikon STORM system uses precision localization technology to achieve up to ~25 nm resolution in xy and 50nm in z over a range of 600nm. The system can be used for 2D or 3D STORM and is compatible with photoswitchable dye pairs or photoswitchable fluorescent proteins.
    Please contact Alison or Kaye about the super-resolution systems.
  2. We have added a confocal stereology package (Visiopharm) to one of our LSM 510 inverted confocal systems.

    Please talk to Kaye about stereology-based assays.
  3. Two new confocal microscopes have recently been installed in the BIRC - a Zeiss LSM 780 system and a Leica TCS SP5 II system. The Leica system replaced two of our oldest confocal microscopes.

    Please ask any of the BIRC staff about the capabilities of the new confocal systems.
  4. The Olympus multiphoton system has been upgraded to a dual IR-laser system, permitting simultaneous photoactivation or photobleaching using one excitation wavelength and imaging using a different IR excitation wavelength.

    Please contact Shiva for more information regarding the multiphoton capabilities of the BIRC.